Pti 428

Manufactured by MedChemExpress

PTI-428 is a laboratory equipment product offered by MedChemExpress. It is a compact and versatile device designed for various research applications. The core function of PTI-428 is to perform precise and reliable measurements and analysis. The detailed specifications and intended use of this product are not available within the scope of this request.

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Lab products found in correlation

Cftrinh 172, by Selleck Chemicals (2 mentions) Vx 809, by Selleck Chemicals (1 mentions) Glpg1837, by Selleck Chemicals (1 mentions) Vx 445, by MedChemExpress (1 mentions) Vx 770, by Selleck Chemicals (1 mentions) Dulbecco s phosphate buffered saline, by Euroclone (1 mentions)

2 protocols using pti 428

Screening for CFTR Modulators

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VX-809, VX-770, GLPG1837 and CFTR_inh-172 were purchased from Selleck Chemicals (Boston, MA). VX-445 and PTI-428 were purchased from MedChemExpress (Monmouth Junction, NJ). Potentiators P2, P3 and P5 are contained in an in-house repository (19 (link)). For screening, a collection of ~50,000 compounds containing diverse drug-like synthetic small molecules (ChemDiv Inc., San Diego, CA) and an in-house repository of mutant CFTR modulators was tested. Other chemicals were purchased from MilliporeSigma (Burlington, MA).

Phuan P.W., Haggie P.M., Tan J.A., Rivera A.A., Finkbeiner W.E., Nielson D.W., Thomas M.M., Janahi I.A, & Verkman A.S. (2020). CFTR modulator therapy for cystic fibrosis caused by the rare c.3700A>G mutation. Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, 20(3), 452-459.

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Optimizing CF Cell Culture Treatments

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SMG1 inhibitor (SMG1i) was provided by the Cystic Fibrosis Foundation (CFF) and dissolved in dimethyl sulfoxide. ELX-02 disulfate (HY-114231B) was purchased from MedChemExpress (Monmouth Junction, NJ, USA). We weighed the precise quantity of ELX-02 powder for every experiment and dissolved it directly in the culture medium to reach a final concentration of 0.5 mg/mL. G418 Sulphate Powder (Euroclone, Pero, Italy) was dissolved in Dulbecco’s phosphate-buffered saline (Euroclone) at a concentration of 50 mg/mL, filtered and stored at +4 °C. For cell treatment, G418 stock was diluted in the medium to a final concentration of 0.5 mg/mL. VX-809 (Lumacaftor), VX-445 (Elexacaftor), and PTI-428 were purchased from MedChemExpress. VX-770 (Ivacaftor) and CFTR_inh-172 were purchased from Selleckchem (Houston, TX, USA). All of these compounds were dissolved in DMSO at a concentration of 10 mM. All other chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Venturini A., Borrelli A., Musante I., Scudieri P., Capurro V., Renda M., Pedemonte N, & Galietta L.J. (2021). Comprehensive Analysis of Combinatorial Pharmacological Treatments to Correct Nonsense Mutations in the CFTR Gene. International Journal of Molecular Sciences, 22(21), 11972.

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