Images were obtained using either a Canon EOS 77D or Canon EOS RP camera with an LMscope adapter (Micro Tech Labs) on either of two Olympus BX-51 fluorescence microscopes equipped with DIC optics. Images were processed for contrast and colour uniformly across images using Adobe Photoshop.
Eos rp
Manufactured by Canon
Sourced in Japan
The EOS RP is a full-frame mirrorless camera from Canon. It features a 26.2-megapixel CMOS sensor, DIGIC 8 image processor, and a 4,779-point Dual Pixel CMOS AF system. The camera can shoot up to 5 frames per second and is capable of recording 4K video at 24 frames per second.
Automatically generated - may contain errors
Lab products found in correlation
11 protocols using eos rp
1
Fluorescence Microscopy Imaging Protocol
2
Contact Angle Measurement of Scaffolds
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Contact angle tests, (ASTM D5725-99/2008, American Society for Testing and Materials, West Conshohocken, Pennsylvania, U.S) were performed on all four scaffold groups. Samples of 10 mm × 10 mm were placed on slides and 10 µL drops of deionized water were deposited on the surface. Images were obtained with a digital camera Canon eos rp, Lens: Canon rf 24–105 mm, f4 (Canon Inc., Melville, NY, USA) Contact angles were calculated using the public domain image analysis software Image J (National Institutes of Health).
3
Thermal Analysis of Dental Composite Restorations
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All the used materials and equipment of this study were collected in Table 1 .
The used materials and equipment in this study
| Name of the material/equipment | Manufacturer |
|---|---|
| Iwanson-caliper | Hager & Werken GmbH & Co., Duisburg, Germany |
| Thermocouple probe (T-type Cu/CuNi) | TC Direct, Budapest, Hungary |
| Flow composite (Filtek Supreme Flowable Restorative) | 3 M, St. Paul, MN, USA |
| Thermal paste (Arctic Silver 5) | Scan Computers International Ltd., Bolton, UK |
| Dental adhesive (Adper Single Bond 2) | 3 M, St. Paul, MN, USA |
| Digital thermometer (EL-EnviroPad-TC) | Lascar Electronics Ltd., Salisbury, UK |
| Non-contact thermometer (Testo845) | Testo Magyarország Kft., Budapest, Hungary |
| 1:5 speed-increasing contra-angle handpiece (TiMax Z95L) | NSK-Nakanishi, Eschborn, Germany |
| Medium-grit diamond cylindrical drill (No. 837) | Hager & Meisinger, Neuss, Germany |
| Dental unit (KaVo Esthetica E30S) | Kaltenbach & Voigt GmbH, Biberach, Germany |
| Digital jewelry scale (SBS-LW-500) | Steinberg Systems, Berlin, Germany |
| Full-frame camera (Canon EOS RP) | Canon, Huntington, USA |
| Prime lens (EF 100 mm f/2.8 L Macro IS USM) | Canon, Huntington, USA |
| Studio flash (Godox MS 300) | Godox Photo Equipment Co., Ltd., Shenzhen, China |
| Softboxes (Godox 90 × 60) | Godox Photo Equipment Co., Ltd., Shenzhen, China |
| Light/lux meter (Voltcraft LX-10) | Conrad, Budapest, Hungary |
| Laptop computer (X1 Carbon sixth gen.) | Lenovo, Beijing, China |
4
Standardized Fish Photography Protocol
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We photographed sexually mature fish inside a plastic tank using a Canon EOS RP with a Macro 100 mm lens (Macro Lens EF 100mm, Canon, Tokyo, Japan), as described (Kwon et al., 2022 (link)). All photos were taken inside a photo studio tent with white light emitting diodes. In each photo, a color card and ruler were visible, and a mirror was placed on the outside of the tank facing the fish to facilitate flaring, allowing for visualization of fins and consistency amongst photos. Before each imaging session, we calibrated the camera using the white balance (CT24-23–1424) on the 24ColorCard (CameraTrax).
5
Colony Formation Assay of Cultured Cells
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Logarithmic growth phase cells were digested with 0.25% trypsin and adjusted to 250 cells/mL. Cells (2 mL/well) were cultured in a 6-well plate at 37 °C and 5% CO2 for 2–3 weeks, and the fresh medium was added every 3 days. Methanol was used to fix the cells, and 1 ml of Giemsa working fluid (48,900, Sigma-Aldrich, Shanghai, China) was used to stain the cells for 30 min. After two washes with ultrapure water, filter paper was used to remove the water around the dish, and the cells were imaged by a camera (Eos RP, Canon, Japan).
6
Dental Veneer Color Determination
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For Group E, which served as the control group, the Canon EOS RP and Canon EF100mm f/2.8 L Macro with eLAB's polar eyes filter were used to capture photographs, and the Emulation S. Hein white balance card (Emulation S. Hein, Freiburg im Breisgau, Germany) was applied (Fig. 3 ). The photographs were then sent to the dental technician's computer, on which eLAB prime software (Emulation S. Hein, Freiburg im Breisgau, Germany) was used to determine the tooth color and assign the CIELAB L∗a∗b∗ value. A porcelain veneer was then fabricated according to the determined tooth color.
7
Assessing Superoxide Accumulation in Plant Leaves
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For analysis of ROS accumulation, the O2− in the B03S leaves was stained with NBT (Yuanye, Shanghai, China) according to the methods of Kumar [64 (link),65 (link)]. First, the leaves were cut into 10 cm long samples and immersed in phosphate buffer (pH 7.5) consisting of 2 mM NBT, 42 mM Na2HPO4 and 8 mM NaH2PO4, followed by vacuum infiltration at room temperature for 12 h. The NBT and O2− combined, forming visible blue stains in the leaves. Then, the leaves were decolorized with 100% ethanol for 1 h. Finally, the decolorized leaves were soaked in glycerol and imaged with a digital camera (EOS RP, Canon, Japan) under visible light.
8
Photographing Flaring Fish in Tanks
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We photographed sexually mature fish inside a plastic tank using a Canon EOS RP with a Macro 100 mm lens (Macro Lens EF 100mm, Canon, Tokyo, Japan), as described (Kwon et al., 2022 (link)). All photos were taken inside a photo studio tent with white light emitting diodes. In each photo, a color card and ruler were visible, and a mirror was placed on the outside of the tank facing the fish to facilitate flaring, allowing for visualization of fins and consistency amongst photos. Before each imaging session, we calibrated the camera using the white balance (CT24-23-1424) on the 24ColorCard (CameraTrax).
9
Quantitative Cell Staining Assay
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Logarithmic growth phase cells were digested with 0.25% trypsin and adjusted to 250 cells/mL. Cells (2 mL/well) were cultured in a 6-well plate at 37 °C and 5% CO 2 for 2-3 weeks, and the fresh medium was added every 3 days. Methanol was used to x the cells, and 1 ml of Giemsa working uid (48900, Sigma-Aldrich, Shanghai, China) was used to stain the cells for 30 min. After two washes with ultrapure water, lter paper was used to remove the water around the dish, and the cells were imaged by a camera (Eos RP, Canon, Japan).
10
Quantitative Cell Staining Assay
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Logarithmic growth phase cells were digested with 0.25% trypsin and adjusted to 250 cells/mL. Cells (2 mL/well) were cultured in a 6-well plate at 37 °C and 5% CO 2 for 2-3 weeks, and the fresh medium was added every 3 days. Methanol was used to x the cells, and 1 ml of Giemsa working uid (48900, Sigma-Aldrich, Shanghai, China) was used to stain the cells for 30 min. After two washes with ultrapure water, lter paper was used to remove the water around the dish, and the cells were imaged by a camera (Eos RP, Canon, Japan).
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