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Analytical kits

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Analytical kits are laboratory equipment designed to perform specific chemical or biological analyses. They typically contain pre-measured reagents, buffers, and other necessary components to facilitate the testing process. The core function of analytical kits is to provide a standardized and efficient method for conducting various analyses in a laboratory setting.

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Lab products found in correlation

Nootkatone, by Merck Group (1 mentions) Melamine, by Merck Group (1 mentions)

4 protocols using analytical kits

1

Measuring Liver, Kidney, and Hormone Levels

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The kits used for the detection of liver function were obtained from Stanbio Laboratory, United States. The kidney function and serum electrolytes (sodium, potassium, and chloride) were measured with analytical kits from Bio-Diagnostics, United Kingdom. Ts, luteinizing hormone (LH), follicle stimulating hormone (FSH), and dehydroepiandrosterone sulfate (DHEA-SO4) kits were purchased from BioSource Co., Nivelles, Belgium. The sex hormone binding globulin (SHBG), γ-glutamyl transpeptidase (γ-GT), glucose-6-phosphate dehydrogenase (G6PD), and 3β-hydroxysteroid dehydrogenase (3βHSD) kits were obtained from IBL Co., Hamburg, Germany. BPA (purity = 99%) was obtained from Sigma-Aldrich, United States, while FA (purity = 98.2%) was purchased from Riven International PVT, LTD, India.
, & Koriem K.M. (2022). Fertaric acid amends bisphenol A-induced toxicity, DNA breakdown, and histopathological changes in the liver, kidney, and testis. World Journal of Hepatology, 14(3), 535-550.
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2

Biochemical and Oxidative Cascade Analysis

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Melamine and nootkatone were obtained from Sigma Chemical Company (St. Louis, MO, USA). The analytical kits used to assay the biochemical parameters and oxidative cascade markers were purchased from Bio-diagnostics Co., Giza, Egypt.
Habotta O.A., Abdeen A., Roomi A.B., Elgndy A.I., Sorour S.M., Morsi M.H., Kamal K.M., Ibrahim S.F., Abdelrahaman D., Fericean L., Banatean-Dunea I., Ghamry H.I., El-Nablaway M., Atawia R.T, & Abdelhady D. (2023). Nootkatone Mitigated Melamine-Evoked Hepatotoxicity by Featuring Oxidative Stress and Inflammation Interconnected Mechanisms: In Vivo and In Silico Approaches. Toxics, 11(9), 784.
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3

Blood Metabolic Profile Analysis

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Three birds from each replicate were taken randomly at 12 weeks of age then slaughtered and blood samples (3 mL/tube) were collected. Later, the blood samples were centrifuged at 4000 roll per minute (rpm) at 4 C for 20 min. Hemolysis-free serum samples were transferred to 1.5 mL micro centrifuge tubes and stored at -20 o C. Albumin, alanine aminotransferase (ALT), aspartate amino transferase (AST), uric acid, blood urea, creatinine, total cholesterol, LDL-and HDL-cholesterol, triglycerides (TG), plasma glutathione (GSH), total antioxidant capacity (T-AOC) and growth hormone (GH) were measured using commercial kits. All blood examinations were done by using analytical kits produced by Bio-diagnostic Cairo, Egypt, www.bio-diagnostic.com).
Salim I., Attia K., Yassin D., El-Atty H.A., & El-Slamony A. (2021). Effect of Low Protein Diets Supplemented with Glycine on Growth Performance Carcass Traits, Blood Parameters and Antioxidant Status of Mandarah Chicks During Starter and Grower Periods. Journal of Animal and Poultry Production, 12(12), 401-408.
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4

Comprehensive Blood Biomarker Analysis

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During the last day of experiment 4 blood samples were collected from each replicate, where 3 blood samples collected in heparinized tubes and the last one of blood sample collected in nonheparinized tube. The 1 st sample centrifuged at 3500 rpm for 15 minute then plasma collected and stored at 20ºC to estimate total cholesterol, LDLcholesterol, HDL-cholesterol, triglycerides, glutathione reductase, total antioxidant capacity (TAOC) and malnodialdhyde (MDA) by colorimetric methods. The 2 nd sample used to determine blood hematological parameters, but the 3 rd sample from each treatment group sent directly to determine activity of glutathione peroxidase activity (GSH-Px) in red blood cells. All blood examinations were done by using analytical kits produced by Bio diagnostic Company.
youssef S., Radwan N.L., Selim N., & Abdlatif H.A. (2021). EFFECT OF SUPPLEMENTED SOME ANTIOXIDANTS TO BROILER DIETS ON GROWTH PERFORMANCE, IMMUNE RESPONSE AND ANTIOXIDANT STATUS DURING SUMMER SEASON. Egyptian Poultry Science, 41(1), 93-111.
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