C57BL/6 J wild-type mice (age of 6–8 weeks) were fed on a high-fat diet (Bio-Serv) for 16 weeks. Control mice received a normal diet (Bio-Serv). On a caloric basis, the high-fat diet consisted of 36% fat, 35.7% carbohydrate, and 20.5% protein (total calories: 5.49 kcal/g). The normal diet contained 5.1% fat, 65.2% carbohydrate, and 18.1% protein (total calories: 3.79 kcal/g). At the end of each trial, body weight was measured and blood glucose levels were assessed with a Roche Accu-Chek Aviva Plus blood glucose meter. These measurements were obtained following a 12 h overnight fast.
Accu chek aviva plus blood glucose meter
Manufactured by Roche
The Accu-Chek Aviva Plus is a blood glucose meter designed for self-monitoring of blood glucose levels. It provides fast and accurate results with a small blood sample size. The device features a user-friendly interface and integrated data management capabilities.
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2 protocols using accu chek aviva plus blood glucose meter
1
High-fat diet induced metabolic changes
2
Murine Submental Blood Glucose and Glutamine
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About 0.3 ml blood was collected by quick sub-mandibular bleeding from mice before sacrifice. Non-fasting blood glucose concentration was measured using the Roche Accu-Chek Aviva Plus Blood Glucose Meter. For glutamine assays, blood samples were deproteinized immediately using the Deproteinizing Sample Preparation Kit from BioVision following the manufacture’s protocol. Deproteinized samples were kept in the −80° freezer before assay. Blood glutamine concentration was measured using EnzyChrom™ Glutamine Assay Kit from BioAssay Systems following the manufacturer’s protocol.
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