Plan apochromat 100 1.4 oil immersion lens

Manufactured by Zeiss

Sourced in Germany

The Plan-Apochromat × 100/1.4 oil immersion lens is a high-performance objective lens manufactured by Zeiss. It is designed to provide exceptional optical performance for microscopy applications. The lens features a magnification of 100x and a numerical aperture of 1.4, which enables the capture of high-resolution, detailed images. The Plan-Apochromat design ensures flat field correction and excellent color correction across the entire field of view.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Axioscope 2 microscope, by Zeiss (1 mentions) Axiocam mrm ccd camera, by Zeiss (1 mentions) Axiovision software, by Zeiss (1 mentions) Axio imager z2 fluorescent microscope, by Zeiss (1 mentions) Zen lite 2011, by Zeiss (1 mentions)

3 protocols using plan apochromat 100 1.4 oil immersion lens

Fungal Morphological Characterization Protocol

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Axenic cultures were grown on synthetic nutrient-poor agar (SNA; Nirenburg 1981) amended with 1-cm² sterile filter paper and carnation leaf pieces, and on PDA as described by Cabral et al. (2012a) . Gross morphological characteristics were studied by mounting the fungal structures in 85 % lactic acid and 30 measurements were made for all taxonomically informative characters at ×1000 magnification using a Plan-Apochromat × 100/1.4 oil immersion lens (Carl Zeiss, Germany) mounted on a Zeiss Axioscope 2 microscope, with differential interference contrast (DIC) illumination. The 95 % confidence levels were determined for the conidial measurements with extremes given in parentheses. For all other fungal structures measured, only the extremes are provided. Colony colour was assessed using 7-d-old cultures on PDA incubated at room temperature and the colour charts of Rayner (1970) . All descriptions, illustrations and nomenclatural data were deposited in MycoBank (Crous et al. 2004 ). Optimal and cardinal growth temperatures were determined by inoculating 90 mm diam PDA plates with a 4 mm diam plug cut from the edge of an actively growing colony. Each isolate was incubated at 4, 10, 15, 20, 25, 30, and 35 °C with three replicate plates per strain at each temperature. Colony diameter of each isolate was determined after 1 wk by measuring the orthogonal directions.

Aiello D., Polizzi G., Crous P.W, & Lombard L. (2017). Pleiocarpon gen. nov. and a new species of Ilyonectria causing basal rot of Strelitzia reginae in Italy. IMA Fungus, 8(1), 65-76.

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Fluorescent Microscopy of Malaria Parasites

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Thin smears of parasite-infected blood, spots of purified sporozoites, and oocyst-attached mosquito midguts were prepared on glass slides, stained with Hoechst 33342, and fluorescent and differential interference contrast (DIC) images were captured using an AxioCam MRm CCD camera (Carl Zeiss, Germany) fixed to an Axio imager Z2 fluorescent microscope with a Plan-Apochromat 100 ×/1.4 oil immersion lens (Carl Zeiss) and Axiovision software (Carl Zeiss). Obtained images were processed using Zen lite 2011 software (Carl Zeiss) and Adobe Photoshop CS (Adobe Systems Inc., San José, CA).
The segregation efficiency of the plasmid per nuclear division was calculated using the following equation:

s e g r e g a t i o n e f f i c i e n c y = 100 \times {(P g f p / 100)}^{1 / n}

, where P_gfp is the percentage of GFP-positive parasites after PYR removal and n is the number of nuclear divisions. In this study, we assumed that 3.5 rounds of nuclear division (equivalent to the generation of 11 daughter parasites) occurred during one blood-stage schizogony of 18 hours.

Thawnashom K., Kaneko M., Xangsayarath P., Chaiyawong N., Yahata K., Asada M., Adams J.H, & Kaneko O. (2019). Validation of Plasmodium vivax centromere and promoter activities using Plasmodium yoelii. PLoS ONE, 14(12), e0226884.

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FM4-64 Uptake Microscopy Assay

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Microscopic analysis of FM4-64 uptake was carried out by a modification of the assay described by Bonhivers and coworkers (Bonhivers et al., 2008 (link)). The modifications included a total of 2.4 × 10⁷ induced (6 days) and non-induced TbSmee1 cKOs harvested by centrifugation, washed in PBS, then resuspended in 3 mL of PBS, 0.1 mM adenosine, and 10 mM glucose. The cells also either remained at 4 °C or were shifted to 27 °C for 10 minutes. To prepare the cells for live imaging, they were washed twice in ice-cold PBS containing 0.1 mM adenosine, 10 mM glucose, and 8 µM of Hoechst 33342 (Thermo Fisher). Then, the cells were resuspended in 100 µL the above buffer without the addition of Hoechst 33342 and were kept on ice until ready to image.
For live imaging, 10 µl of cell suspension was spotted onto a glass slide and a coverslip was laid on top prior to visualization with a Zeiss Observer Z1 equipped with a Hamamatsu Orca Flash 4.0 camera (Hammamatsu) and a Plan-Apochromat 100×/1.4 oil immersion lens (Zeiss).

Perry J.A., Sinclair-Davis A.N., McAllaster M.R, & de Graffenried C.L. (2017). TbSmee1 regulates hook complex morphology and the rate of flagellar pocket uptake in Trypanosoma brucei. Molecular microbiology, 107(3), 344-362.

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