Lumipulse g sars cov 2 ag assay

Manufactured by Fujirebio

Sourced in Japan

The Lumipulse G SARS-CoV-2 Ag assay is an automated chemiluminescent enzyme immunoassay designed for the qualitative detection of SARS-CoV-2 nucleocapsid antigen in human nasopharyngeal and oropharyngeal swab specimens.

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Lab products found in correlation

Loopamp sars cov 2 detection kit, by Eiken Chemical (1 mentions) Lumipulse g system, by Fujirebio (1 mentions)

2 protocols using lumipulse g sars cov 2 ag assay

SARS-CoV-2 Antigen Testing in Healthcare Setting

Cited 2 times

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Case definition included all healthcare workers and all patients associated to the ward from January 27 to January 31, 2022 with a positive SARS-COV-2 antigen test using the Lumipulse G SARS-CoV-2 Ag assay (Fujirebio, Tokyo, Japan), which detects the SARS-CoV-2 nucleocapsid (N) protein [9 (link)]. Non-case definition included all healthcare workers and all patients associated to the ward from January 27 to January 31, 2022 with a negative SARS-CoV-2 antigen test. Cases with judgment-pending results in Lumipulse G were confirmed using the Loopamp™ SARS-CoV-2 Detection Kit (Eiken Chemical, Tokyo, Japan) [10 (link)], which targets genes encoding the nucleocapsid (N) and the RNA-dependent RNA polymerase of SARS-CoV-2, or the ID NOW COVID-19 assay (Abbott Rapid Diagnostic, Scarborough, ME, USA), which uses isothermal nucleic acid amplification of RNA-dependent RNA polymerase viral targets [11 (link),12 (link)]. Saliva or nasopharyngeal swab specimens were obtained from all members of the ward (including all healthcare workers and patients). The type of variant strain was not evaluated by laboratory methods.

Itoh N., Akazawa N., Ishikane M., Kawabata T., Kawamura D., Chikusa T., Kodama E.N, & Ohmagari N. (2022). Lessons learned from an outbreak of COVID-19 in the head and neck surgery ward of a Japanese cancer center during the sixth wave by Omicron. Journal of Infection and Chemotherapy, 28(12), 1610-1615.

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Detecting SARS-CoV-2 in Breastmilk

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Breastmilk samples were centrifuged (twice for 5 minutes each at 2000g), and approximately 200 μL of the resulting supernatant was used for detection and quantitation of SARS-CoV-2 nucleocapsid protein antigens via a chemiluminescent enzyme immunoassay (Lumipulse G SARS-CoV-2 Ag assay with the LUMIPULSE G System; Fujirebio). To avoid false-negative results, an internal control (10 μL) obtained from breastmilk samples of 7 control mothers who were breastfeeding was resuspended with supernatant (1 mL). Antigenic tests were also performed using these samples.

Conti M.G., Terreri S., Piano Mortari E., Albano C., Natale F., Boscarino G., Zacco G., Palomba P., Cascioli S., Corrente F., Capponi C., Mirabella M., Salinas A.F., Marciano A., De Luca F., Pangallo I., Quaranta C., Alteri C., Russo C., Galoppi P., Brunelli R., Perno C.F., Terrin G, & Carsetti R. (2021). Immune Response of Neonates Born to Mothers Infected With SARS-CoV-2. JAMA Network Open, 4(11), e2132563.

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