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Pe cy7 rat anti mouse cd45 clone 30 f11

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PE-Cy7 rat anti-mouse CD45 (clone 30-F11) is a fluorescently-labeled antibody that binds to the CD45 antigen on mouse cells. CD45 is a transmembrane glycoprotein expressed on the surface of all leukocytes.

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Lab products found in correlation

Facsaria 2 sorp, by BD (2 mentions) Streptavidin pe, by BD (2 mentions) Pe cy7 rat anti mouse cd31 clone 390, by BD (2 mentions) Biotin rat anti mouse cd106 clone 429, by BD (2 mentions) Apc anti mouse human cd11b clone m1 70, by BioLegend (1 mentions) Pe rat anti mouse siglec f clone e50 2440, by BD (1 mentions) Fitc anti mouse cd11c clone n418, by BioLegend (1 mentions)

Close spelling variants of this product

PE-Cy7 anti-mouse CD45 (clone 30-F11; Rat anti-mouse CD45 (clone 30-F11, Anti-CD45-PE (clone: 30-F11) Rat anti-mouse CD45 (30-F11, Anti-mouse CD45 (clone 30-F11, CD45-PE (clone 30-F11; Anti-CD45 (clone 30-F11, PE/Cy7 Rat Anti-Mouse CD45 Anti-mouse CD45 (30-F11, CD45 (clone 30-F11,

3 protocols using pe cy7 rat anti mouse cd45 clone 30 f11

1

Satellite Cell Identification in Muscle

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Muscle samples were stained using an antibody mixture of PE-Cy7 rat anti-mouse CD31 (clone 390), PE-Cy7 rat anti-mouse CD45 (clone 30-F11), Biotin rat anti-mouse CD106 (clone 429(MVCAM.A)) and PE Streptavidin from BD Biosciences (San Diego, CA); and Itga7 647 (clone R2F2) from AbLab (Vancouver, B.C., Canada). Antibody cocktail was added to samples and incubated on ice for 30 min. Samples were washed and resuspended with FACS staining media containing propidium iodide for FACS analysis on the FACSAriaII SORP (BD Biosciences, San Diego, CA). Total satellite cells (lineage negative; VCAM, alpha7 double positive cells or ZsGreen+) (Figures S1A and S1B) were analyzed while draining the entire sample from each skeletal muscle sample. For transplanted tibialis anterior (TA) muscles, the number of donor (ZsGreen + or tdTOM+) satellite cells were examined as previously described (Arpke et al., 2013 (link)).
Collins B.C., Arpke R.W., Larson A.A., Baumann C.W., Xie N., Cabelka C.A., Nash N.L., Juppi H.K., Laakkonen E.K., Sipilä S., Kovanen V., Spangenburg E.E., Kyba M, & Lowe D.A. (2019). Estrogen Regulates the Satellite Cell Compartment in Females. Cell reports, 28(2), 368-381.e6.
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2

Satellite Cell Identification in Muscle

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Muscle samples were stained using an antibody mixture of PE-Cy7 rat anti-mouse CD31 (clone 390), PE-Cy7 rat anti-mouse CD45 (clone 30-F11), Biotin rat anti-mouse CD106 (clone 429(MVCAM.A)) and PE Streptavidin from BD Biosciences (San Diego, CA); and Itga7 647 (clone R2F2) from AbLab (Vancouver, B.C., Canada). Antibody cocktail was added to samples and incubated on ice for 30 min. Samples were washed and resuspended with FACS staining media containing propidium iodide for FACS analysis on the FACSAriaII SORP (BD Biosciences, San Diego, CA). Total satellite cells (lineage negative; VCAM, alpha7 double positive cells or ZsGreen+) (Figures S1A and S1B) were analyzed while draining the entire sample from each skeletal muscle sample. For transplanted tibialis anterior (TA) muscles, the number of donor (ZsGreen + or tdTOM+) satellite cells were examined as previously described (Arpke et al., 2013 (link)).
Collins B.C., Arpke R.W., Larson A.A., Baumann C.W., Xie N., Cabelka C.A., Nash N.L., Juppi H.K., Laakkonen E.K., Sipilä S., Kovanen V., Spangenburg E.E., Kyba M, & Lowe D.A. (2019). Estrogen Regulates the Satellite Cell Compartment in Females. Cell reports, 28(2), 368-381.e6.
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3

Multiparameter Flow Cytometry Profiling

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PE-Cy7 rat anti-mouse CD45 clone 30-F11, PE rat anti-mouse Siglec-F clone E50-2440, Alexa Fluor 647 rat anti-mouse CD193 (CCR3), and their relevant isotype controls were purchased from BD Biosciences. FITC anti-mouse CD11c clone N418, APC anti-mouse/human CD11b clone M1/70, APC anti-mouse CD16/32 clone 93, APC anti-mouse FcεRIα clone MAR-1, APC anti-mouse CD23 clone B3B4, APC anti-mouse CD64 clone X54-5/7.1, and their relevant isotype controls were purchased from Biolegend (San Diego, CA). Aqua fluorescent reactive dye for live/dead labeling was purchased from Life Technologies.
Smith K.M., Rahman R.S, & Spencer L.A. (2016). Humoral immunity provides resident intestinal eosinophils access to luminal antigen via eosinophil-expressed low affinity Fc gamma receptors. Journal of immunology (Baltimore, Md. : 1950), 197(9), 3716-3724.
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