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Penicillin streptomycin

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Penicillin/streptomycin is a commonly used antibiotic solution that provides broad-spectrum antimicrobial activity. It is a sterile, cell culture-tested mixture of penicillin and streptomycin sulfate.

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Lab products found in correlation

Fetal bovine serum (fbs), by Atlanta Biologicals (21 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (9 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions) Penicillin, by Thermo Fisher Scientific (5 mentions) Streptomycin, by Thermo Fisher Scientific (5 mentions) Horse serum, by Atlanta Biologicals (4 mentions) Hydrocortisone, by Merck Group (3 mentions) Cholera toxin, by Merck Group (3 mentions) Mda mb 231 cell, by American Type Culture Collection (2 mentions) Insulin, by Merck Group (2 mentions) Dulbecco modified eagle medium (dmem), by Atlanta Biologicals (2 mentions) Penicillin, by Atlanta Biologicals (2 mentions) Streptomycin, by Atlanta Biologicals (2 mentions) Hydrogen peroxide, by Thermo Fisher Scientific (2 mentions) Bovine serum albumin fraction 5 bsa, by Rockland Immunochemicals (2 mentions) Sephadex g 25 fine resin, by GE Healthcare (2 mentions) Anti cd3, by Cytek Biosciences (2 mentions) Fabian automated enrichment system, by IBA Lifesciences (2 mentions) Cd4 isolation kit, by IBA Lifesciences (2 mentions)

30 protocols using penicillin streptomycin

1

Culturing Breast Cancer Cell Lines

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MCF-10A cells (ATCC) were cultured in DMEM/F12 medium (Invitrogen) with 5% horse serum (Atlanta Biologicals, Inc.), 1% penicillin/streptomycin (Invitrogen), and 20ng/ml EGF, 0.5mg/ml hydrocortisone, 100ng/ml cholera toxin, 10 μg/ml insulin (all were from Sigma). MCF-7 cells (ATCC) were cultured in Eagle’s MEM (Invitrogen), 10% fetal bovine serum (FBS, Atlanta Biologicals, Inc.), 10μg/ml insulin, and 1% penicillin/streptomycin. MDA-MB-231 cells (ATCC) were cultured in alpha MEM with 10% FBS and 1% penicillin/streptomycin.
He Q., Gong Y., Gower L., Yang X, & Friesel R.E. (2016). Sef Regulates Epithelial-Mesenchymal Transition in Breast Cancer Cells. Journal of cellular biochemistry, 117(10), 2346-2356.
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2

Characterization of Breast and Bone Cancer Cell Lines

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MCF7 cells are human breast adenocarcinoma cells derived from human mammary tissue (ATCC-HTB-22). These cells were maintained in RPMI medium (GIBCO) supplemented with 10% fetal bovine serum (FBS) (Gemini-Bio), 1% penicillin-streptomycin (GIBCO). U2OS cells are immortalized bone cancer cells (ATCC-HTB-96). These cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO) supplemented with 10% FBS and 1% penicillin-streptomycin. U2OS EJ-DR cells were maintained in DMEM, 10% Tetracyline-free FBS (Atlanta Biologicals) and 1% penicillin-streptomycin. All the cell lines were grown at 37°C in a 5% CO2 humidified incubator. DNA-PKcs inhibitors NU7441 and KU0060648 and triamcinolone (TA) ligand were kindly provided by Dr Ranjit Bindra, Yale University.
Ray S., Breuer G., DeVeaux M., Zelterman D., Bindra R, & Sweasy J.B. (2017). DNA polymerase beta participates in DNA End-joining. Nucleic Acids Research, 46(1), 242-255.
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3

Culturing MCF-10A and MDA-MB-231 Cell Lines

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MCF-10A cells were grown in DMEM/F12 (Corning: 10090CV) supplemented with 5% horse serum (Gibco: 16050), 10 ug/ml human insulin (Sigma Aldrich: I-1882), 20 ng/ml recombinant hEGF (Peprotech: AF-100-15), 100 ng/ml cholera toxin (Sigma: C-8052), 0.5 ug/ml hydrocortisone (Sigma: H-0888), 1% penicillin/streptomycin (Life Technologies: 15140-122), and 2 mM glutamine (Life Technologies: 25030-081). MDA-MB-231 cells were grown in DMEM/F12 supplemented with 10% fetal bovine serum (Atlanta Biologicals, S11550), 1% penicillin/streptomycin, and 2 mM glutamine.
Tracy K.M., Tye C.E., Ghule P.N., Malaby H.L., Stumpff J., Stein J.L., Stein G.S, & Lian J.B. (2018). Mitotically-associated lncRNA (MANCR) Affects Genomic Stability and Cell Division in Aggressive Breast Cancer. Molecular cancer research : MCR, 16(4), 587-598.
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4

Culturing Primary Human Endothelial and Immune Cells

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Primary human neonatal dermal microvascular cells (HNDMVECs, CC-2516) were purchased from Lonza (Walkersville, MD) and cultured in endothelial cell growth medium MV2 (ECG MV2, C-22121) (PromoCell, Heidelberg, Germany) and 100U/mL penicillin-streptomycin (Gibco, Waltham, MA). A HEK293T cell line was purchased from Invitrogen (Waltham, MA) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Waltham, MA) with 10% fetal bovine serum (FBS) (Atlanta Biologicals, Flowery Branch, GA) and 100U/mL penicillin-streptomycin. The medium was changed every 2 days and the cells were harvested by trypsinization. The human acute leukemia monocytic cell line THP-1 (TIB-202) was purchased from American Type Culture Collection (ATCC, Manassas, VA) and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (ATCC Modification) (Gibco, Waltham, MA) with 10% FBS. The medium was changed every 2 days. Cells were rinsed with fresh medium twice after pretreatment.
Zhou H., Tu L.N., Giachelli C., Nigam V, & Scatena M. (2023). Monocyte Adhesion and Transmigration Through Endothelium Following Cardiopulmonary Bypass Shearing is Mediated by IL-8 Signaling. bioRxiv.
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5

Panc-1 PDAC Cell Line Stimulation

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Human PDAC cell line (Panc‐1 ATCC® CRL‐1469™) was purchased from the ATCC. Panc‐1 cells were plated in Dulbecco's Modified Eagle Medium (DMEM) basic (1×) (Gibco). DMEM was supplemented by 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (ATLANTA Biologicals). Next, cells were collected and washed twice with phosphate‐buffered saline (PBS). Then, 1 × 106 cells/well were cultured in a 48‐well plastic plate (Corning Costar). Thereafter, cell culture medium was supplemented by 1 µg/mL l‐Tryptophan (Sigma‐Aldrich) and incubated for 72 hours. A total of 20 ng/mL of human purified recombinant indoleamine 2,3 dioxygenase1 (hIDO1) (Prospec) was added every 6 hours to stimulate the Kynp in the tumor cells. This culture system was duplicated and repeated three times.
Alahdal M., Sun D., Duan L., Ouyang H., Wang M., Xiong J, & Wang D. (2021). Forecasting sensitive targets of the kynurenine pathway in pancreatic adenocarcinoma using mathematical modeling. Cancer Science, 112(4), 1481-1494.
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6

DT40 Cell Line DNA Repair Mutants

Cited 1 time
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Fetal bovine serum (FBS) and penicillin/streptomycin were obtained from Atlanta Biologicals (Norcross, GA, USA) and Sigma-Aldrich (St. Louis, MO, USA), respectively. RPMI 1640 culture medium (+glutamine, −phenol red) and chicken serum (CS) were acquired from Life Technologies (Grand Island, NY, USA). FBS and CS were heat inactivated at 56°C for 30 min. DT40 cells were maintained as described in our previous report [13 (link)]. The DT40 cell line and its isogenic mutants were knocked out in specific DNA repair pathways (Supplementary Table S1).
Hashimoto K., Sharma V., Sasanuma H., Tian X., Takata M., Takeda S., Swenberg J.A, & Nakamura J. (2016). Poor recognition of O6-isopropyl dG by MGMT triggers double strand break-mediated cell death and micronucleus induction in FANC-deficient cells. Oncotarget, 7(37), 59795-59808.
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7

Isolation and Characterization of Human Mesenchymal Stem Cells

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Human MSCs were obtained from healthy donors via iliac crest bone marrow biopsy, then cultured in Minimum Essential Medium Eagle, Alpha Modification (#SH30265.01; GE Health Life Sciences, Chicago, IL) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (#S11150H and #B21110; Atlanta Biologicals, Atlanta, GA). All studies involving bone marrow-derived human MSCs were approved by the Emory University Institutional Review Board and were in accordance with institutional guidelines. MSCs were plated on 75 cm tissue culture flasks (#H353133; Corning, Corning, NY) and maintained in culture at 37°C in the presence of 5% carbon dioxide. Only cell passages 2–5 were used to conduct experiments. The phenotypic markers CD73, CD90, and CD105 were confirmed by flow cytometry. MSCs for co-culture experiments were grown to 80% confluence then seeded onto 96-well plates (Corning) at a density of 5 × 103 cells/well. Some MSCs were treated for 12 hours with and without 100μM of the irreversible CD73 inhibitor, adenosine 5′ (α, β-methylene) diphosphate sodium salt (APCP; #363310; Tocris, Minneapolis, MN).
Xu K., Cooney K.A., Shin E.Y., Wang L., Deppen J.N., Ginn S.C, & Levit R.D. (2019). Adenosine from a biologic source regulates neutrophil extracellular traps (NETs). Journal of leukocyte biology, 105(6), 1225-1234.
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8

Synergistic Effects of Heavy Metal and Dioxin Exposure

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BEAS-2B was cultured in Dulbecco’s Modified Eagle’s Medium
(Cellgro) supplemented with 1% penicillin streptomycin and 10% fetal
bovine serum (Atlanta Biologicals) at 37 °C and 5% CO2. For Hg
and TCDD exposures, the cells were treated with different doses of HgCl2 (0,
0.5 and 2.5 μM) and TCDD (0, 2 and 10 nM), either individually or in combination
(Hg 0.5 μM/TCDD 2.0 nM; Hg 2.5 μM/TCDD 10 nM) for 3 weeks. The cells were
split at 80% confluence. The cell culture medium was changed every 48 h and
appropriate amounts of fresh Hg and TCDD were added to the cells. HgCl2 was
obtained from Sigma (215465) and 2,3,7,8-tetrachlorodibenzo-p-dioxin
(TCDD) was obtained from AccuStandard (D-404S).
Jagannathan L., Jose C.C., Tanwar V.S., Bhattacharya S, & Cuddapah S. (2017). Identification of a unique gene expression signature in mercury and 2,3,7,8-tetrachlorodibenzo-p-dioxin co-exposed cells †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6tx00432f. Toxicology Research, 6(3), 312-323.
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9

hMSC Mineral Concentration Screening

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All experiments were performed with hMSCs passage 5 or lower, and cells were cultured in normal media [α-modified minimal essential media (AMEM); HyClone], 16.5% fetal bovine serum (FBS; Atlanta Biologicals), and 1% penicillin/streptomycin (Gibco), unless otherwise stated. Mineral solutions were prepared using lithium chloride (LiCl), magnesium sulfate (MgSO4), and sodium silicate (Na2O3Si) with concentrations between 0 and 10 mg/ml. hMSCs were seeded in 96-well plates at a density of 10,000 cells/cm2 and after 24 hours were subjected to various mineral concentrations for an additional 24 hours. Minerals were then removed, and an MTT assay (the American Type Culture Collection) was performed according to the manufacturer’s protocol. In addition, once desired concentrations were determined, an alamarBlue assay (Thermo Fisher Scientific) was performed at 1, 3, 7, and 14 days to quantify metabolic activity. For both MTT and alamarBlue, control groups consisted of untreated cells (negative) and cells treated with nSi (positive).
Brokesh A.M., Cross L.M., Kersey A.L., Murali A., Richter C., Gregory C.A., Singh I, & Gaharwar A.K. (2022). Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program. Science Advances, 8(17), eabl9404.
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10

Cytokine Profiling and Apoptosis Assessment

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PL (purity 99%), dimethyl sulfoxide (DMSO), 0.25% Trypsin-EDTA solution, Alamar Blue® (solution of resazurin fluorescence dye), Taxol® (paclitaxel), and lipopolysaccharides from Escherichia coli O111: B4 (LPS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell culture flasks and plates, Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from VWR International (Radnor, PA, USA). Penicillin/streptomycin and DPBS were obtained from Atlanta Biologicals (Atlanta, GA, USA). Human Cytokine Antibody Array Kit (Cat # AAH-CYT-1000), Human MCP-1 ELISA kit (Cat # ELH-MCP1) and TNF-α were purchased from RayBiotech (Norcross, GA, USA). SsoAdvancedTM Universal SYBR® Green Supermix and NF-қB Signaling Pathway (SAB Target List) H96 were purchased from Bio-Rad (Bio-Rad, Hercules, CA). DNA-free™ Kit (Cat # AM1907) and EnzChek® Caspase-3 Assay were purchased from Life Technologies Inc. (Grand Island, NY, USA).
Messeha S.S., Zarmouh N.O., Mendonca P., Alwagdani H., Kolta M.G, & Soliman K.F. (2018). The inhibitory effects of plumbagin on the NF-қB pathway and CCL2 release in racially different triple-negative breast cancer cells. PLoS ONE, 13(7), e0201116.
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