Calcein acetoxymethyl ester calcein am

MesenCult MSC Basal Medium and MesenCult Stimulatory Supplements were obtained from StemCell Technologies Inc. (Vancouver, Canada). Plasmid DNA coding luciferase enzyme (pGL-3) and Cx43-siRNA was obtained from Sangon Biotechnology Co. Ltd. (Shanghai, China). Plasmid DNA coding for HSV-tk was purchased from InvivoGen Biotechnology Co. (San Diego, USA). GCV was obtained from Haikou Qili Pharmaceutical Co., Ltd. (Hainan, China). 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (CM-Dil) was obtained from Invitrogen Life Technologies (Carlsbad, USA). Calcein acetoxymethyl ester (calcein-AM), 1% crystal violet and 4',6-diamidino-2-phenylindole (DAPI) were purchased from Yeasen Biotechnology Co. Ltd. (Shanghai, China). Dulbecco's modified Eagle's medium (DMEM) and foetal bovine serum (FBS) were purchased from Gibco BRL (Gaithersburg, USA). Iron (III) acetylacetonate (99%) and 4-biphenylcarboxylic acid (95%) were purchased from Acros Organics (Geel, Belgium). Polycaprolactone (MW = 14,000), oleic acid (90%), and branched polyethylenimine (PEI, MW = 25,000) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, USA). Chloroform and tetrahydrofuran in chemically pure were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).

Pd(acac)₂, Au(PPh₃)Cl, N,N-dimethylacetamide, N,N-dimethylformamide (DMF), polyvinyl pyrrolidone (PVP), NaBr were purchased from the Sigma-Aldrich (Shanghai, China). Cy5.5-NHS and Hoechst were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. SH-mPEG and NH₂-PEG-SH were obtained from Shanghai Ziqi Biotechnology Co., Ltd. Calcein-acetoxymethyl ester (calcein-AM) and propidium iodide (PI) assay kit were obtained from Yeasen Biotechnology (Shanghai) Co., Ltd. All chemicals and reagents used in this work were analytical grade and used without any further purification. The radiopharmaceutical ⁹⁰Y resin microspheres were provided by the First Affiliated Hospital of School of Medicine of Zhejiang University.