Galaxy note 4

Following miniaturization, functionality as a platform for performing chemical assays was confirmed by performing a glucose assay on a miniaturized microPAD with a sample zone, a reagent zone, a test zone and a waste zone all connected in series by a straight channel (Fig. S1)^{41 (link)}. The reagents for the assay were deposited onto the reagent zone using a reagent pencil, which was fabricated by pressing a mixture of 66.6% w/w polyethylene glycol (M_n 2000 g/mol), 22.2% w/w graphite powder, 0.75% w/w glucose oxidase (GOx, 266 U/mg), 0.52% w/w horseradish peroxidase (HRP, 293 U/mg), and 10.0% w/w 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) into the shape of a cylindrical pellet with a diameter of 3.2 mm using a manual pellet press (Parr Instrument Company)^{41 (link),42} . Glucose solutions (3.5 µL) prepared in 1X PBS with concentrations of 0, 0.3, 0.6, 0.9 and 1.2 mM were applied to the sample zone of the device and a colorimetric readout was generated in the test zone. The intensity of the color produced in the test zones was measured via digital image colorimetry (DIC)^{43 (link)}, where the mean color intensity in the red channel of the test zones was measured using a smartphone (Samsung Galaxy Note 4) and the Color Grab application^{9 (link)}.

A Samsung Galaxy Note 4 smartphone was purchased for the imaging of
the RT-LAMP reaction on the microchip substrate. The smartphone hardware was
not modified from its factory conditions. A Thorlabs 530 nm Longpass Colored
Glass Filter was placed between the camera and the chip to isolate the
fluorophore emission wavelengths. A 3D-printed cradle (Figure 1) was designed to position the smart-phone
horizontally with the camera directly above the microchip. A mounting
cylinder was also 3D printed to hold an Opto Diode Corp high-output blue
light-emitting diode (LED) and a Thorlabs Shortpass Filter with a 500 nm
cut-off wavelength, which fit within the cradle and illuminated the
microchip from an angle. The LED was powered with 3 V from an Agilent E364xA
DC power supply with an automated on/off function controlled with a MATLAB
script. It was also determined that the blue LED could be adequately powered
by a standard 3 V lithium coin battery, but the DC power supply was used for
the purpose of PC control.

Eye tracking glasses (SIMI, version 2.0, SensoMotoric Instruments Inc., Teltow, Germany) recorded binocular eye gaze behavior via a wireless connection to a Samsung Galaxy Note 4 (placed in the climber’s chalk bag) in real time at a sampling rate of 60 Hz, with gaze tracking range of 80° horizontal and 60° vertical, and gaze tracking accuracy of 0.5° over all distances. The scene camera located between the eyes records at 960 x 720 pixels with field of view: 60° horizontal, 46° vertical, and a sampling rate of 30 Hz (www.eyetracking-glasses.com). A three-point calibration was used before each trial, and at the end of each trial the climber fixated the last hold so we could check for any offset due to movement of the glasses during the climb.

For 8 weeks, subjects were provided a Samsung Galaxy Note 4 smartphone that they were instructed to use as their primary phone during the study period. Subjects were encouraged to use their current phone number and subscriber identification module card; with the exception of 1 subject, all subjects did so. During the study period, trained staff at the University of Michigan administered the Structure Interview Guide for the Hamilton Depression Rating Scale (HDRS) [9 (link)] and Young Mania Rating Scale (YMRS) [10 (link)] once a week via phone interviews.