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Flocked nasal swabs

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Sourced in Italy

Flocked nasal swabs are a type of medical device used for the collection of samples from the nasal cavity. They feature a flocked tip designed to efficiently collect and release samples for analysis.

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Lab products found in correlation

Universal transport medium, by Copan (1 mentions) Dneasy blood tissue kit, by Qiagen (1 mentions)

4 protocols using flocked nasal swabs

1

Multiplex PCR for Respiratory Pathogens

Cited 2 times
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Flocked nasal swabs (Copan, Italy) were used to sample turbinate nasal. Samples were assessed for human adenovirus species B-D; human bocavirus; coronaviruses OC43, 229E, HKU1 and NL63; influenza viruses A, B and C; parainfluenzaviruses 1–4; KI and WU polyomaviruses; respiratory syncytial virus types A and B and human metapneumovirus, using a tandem multiplex real-time PCR assay [25] (link), [26] (link).
A PCR directed at picornavirus sequences in the 5′UTR was used to detect RV. The products were identified to species level as RV-A, RV-B or RV-C by sequencing of this 260 bp product and analysed using ClustalX software [27] (link), [28] (link). The local hospital laboratory at Bispebjerg Hospital completed bacterial cultures on spontaneous expectorates collected during the exacerbation [29] .
Bjerregaard A., Laing I.A., Poulsen N., Backer V., Sverrild A., Fally M., Khoo S.K., Barrett L., Baltic S., Thompson P.J., Chidlow G., Sikazwe C., Smith D.W., Bochkov Y.A., Le Souëf P, & Porsbjerg C. (2016). Characteristics associated with clinical severity and inflammatory phenotype of naturally occurring virus-induced exacerbations of asthma in adults. Respiratory Medicine, 123, 34-41.
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2

Intranasal Live Attenuated Influenza Vaccine

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LAIV was administered to participants as a 0.1-mL spray in each nostril after receiving informed consent. Each dose contained approximately 106.5–7.5 fluorescent focus units of each of the 3 recombinant strains for the 2013–2014 influenza season: A/California/7/2009 (H1N1)pdm09-like virus, A/Texas/50/2012-like virus, and B/ Massachusetts/2/2012-like virus [23 ]. Participants were followed for 7 days from the time of vaccination because previous pediatric studies showed that the highest titers of influenza virus shed after LAIV were within this time period [24 (link)–26 (link)]. Flocked nasal swabs (Copan Diagnostics Inc, Murrieta, CA) were taken from participants immediately before vaccination on day 0 (D0) by a trained research nurse. Proper specimen collection technique was taught to participants (and/or their parents), and nasal samples were subsequently self-obtained on days 1, 2, 4, and 7 after vaccination (D1–D7, respectively). Swabs were immediately placed in 2.0 mL of universal transport medium (Copan) and then kept in home freezers and delivered frozen to the hospital sites. The specimens were then stored at −80°C, and all virologic testing was performed at the Laboratoire de Santé Publique du Québec.
Boikos C., Joseph L., Martineau C., Papenburg J., Scheifele D., Lands L.C., De Serres G., Chilvers M, & Quach C. (2016). Influenza Virus Detection Following Administration of Live-Attenuated Intranasal Influenza Vaccine in Children With Cystic Fibrosis and Their Healthy Siblings. Open Forum Infectious Diseases, 3(4), ofw187.
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3

Multiplex PCR for Respiratory Pathogens

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Flocked nasal swabs (Copan, Italy) were used to sample turbinate nasal. Samples were assessed for human adenovirus species B-D; human bocavirus; coronaviruses OC43, 229E, HKU1 and NL63; influenza viruses A, B and C; parainfluenzaviruses 1 – 4; KI and WU polyomaviruses; respiratory syncytial virus types A and B and human metapneumovirus, using a tandem multiplex real-time PCR assay.[25 ,26 (link)] A PCR directed at picornavirus sequences in the 5′UTR was used to detect RV. The products were identified to species level as RV-A, RV-B or RV-C by sequencing of this 260bp product and analysed using ClustalX software[27 ,28 (link)]. The local hospital laboratory at Bispebjerg Hospital completed bacterial cultures on spontaneous expectorates collected during the exacerbation[29 ].
Bjerregaard A., Laing I.A., Poulsen N., Backer V., Sverrild A., Fally M., Khoo S.K., Barrett L., Baltic S., Thompson P.J., Chidlow G., Sikazwe C., Smith D.W., Bochkov Y.A., Le Souëf P, & Porsbjerg C. (2016). Characteristics associated with clinical severity and inflammatory phenotype of naturally occurring virus-induced exacerbations of asthma in adults. Respiratory Medicine, 123, 34-41.
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4

Molecular Detection and Sequencing of JCPyV

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DNA from blood, serum, urine, flocked nasal swabs (Copan Diagnostics, USA) and CSF (200μL of each sample) was extracted using Roche High Pure Viral Nucleic Acid kits (Roche Diagnostics, Australia), while Qiagen DNeasy Blood & Tissue kits (Qiagen, Australia) were used for kidney tissue (approximately 25mg) DNA extraction, as per manufacturers' instructions. Nasal swabs were suspended in 1mL of PBS prior to extraction. Fresh scalpel blades and plasticware were used to collect the different sections of kidney tissue.
Endogenous retrovirus 3 (ERV3)1 (link) was used for cellular quantification. Real-time PCRs specific for BKPyV (assay V3A)2 (link) and JCPyV (assay JL1)3 (link) were used for both initial diagnostic testing and subsequent quantification. Primer targets did not share homology with any of the newly discovered human polyomaviruses. Quantified A549 tissue culture and a 3373bp pMA-RQ(ampR) synthetic plasmid containing the JL1 assay target (Life Technologies, Germany) were used to generate standard curves for quantification of ERV3 and JCPyV, respectively.
JCPyV genomes were generated using PCR with 10 overlapping primer pairs (Supplementary Table 1) and standard Sanger sequencing. Genome assembly and analyses were performed using CLC Main Workbench 6.6.1 (CLC Bio, Denmark).
Bialasiewicz S., Hart G., Oliver K., Agnihotri S.P., Koralnik I.J., Viscidi R., Nissen M.D., Sloots T.P., Burke M.T., Isbel N.M, & Burke J. (2017). A Difficult Decision: Atypical JC Polyomavirus Encephalopathy in a Kidney Transplant Recipient. Transplantation, 101(6), 1461-1467.
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