Cd19 clone hib19

Manufactured by Thermo Fisher Scientific

The CD19 (clone HIB19) is a lab equipment product. It is a monoclonal antibody that binds to the CD19 antigen, which is expressed on the surface of B cells and B cell precursors.

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Lab products found in correlation

Igm clone mhm 88, by BioLegend (2 mentions) Cd27 clone o323, by BioLegend (2 mentions) Igd clone ia6 2, by BioLegend (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Aria 2, by BD (1 mentions) Cd4 clone rpa t4, by BioLegend (1 mentions) Clone j252d4, by BioLegend (1 mentions) Pd 1 clone eh12, by BD (1 mentions) Cd45ro clone uchl1, by BD (1 mentions) Cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Facsaria instrument, by BD (1 mentions) Summit v4, by Beckman Coulter (1 mentions) Cd56 clone af12 7h3, by Miltenyi Biotec (1 mentions) Cyan adp flow cytometer, by Agilent Technologies (1 mentions) Viability stain, by Thermo Fisher Scientific (1 mentions) Lsr fortessa cytometer, by BD (1 mentions) Fc blocked, by BD (1 mentions) Cd38 clone hit2, by BioLegend (1 mentions) Human fc block, by BD (1 mentions) Cd16 clone b73.1, by BioLegend (1 mentions)

Close spelling variants of this product

CD19-APC (clone HIB19), (2 citations) FITC-anti-CD19 (clone HIB19) (2 citations) Anti-human-CD19-eF450 (clone HIB19), (2 citations)

5 protocols using cd19 clone hib19

Sorting Naive and Memory B Cells

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PBMCs were stained with antibodies specific for CD19 (clone HIB19, eBiosciences, San Diego, CA), IgM (clone MHM-88), IgD (clone IA6-2), and CD27 (clone O323, all from BioLegend, San Diego, CA). B cell populations were sorted using an Aria II (BD Biosciences) into naive (CD19+CD27- and IgM+ and/or IgD+) or memory (CD19+CD27+) cells. Purity of sorted populations was tested and confirmed to have <2% contamination by the other B cell subset. Cells were lysed using TRIzol Reagent (Life Technologies, Grand Island, NY) and stored at -80°C until RNA extraction.

Roskin K.M., Simchoni N., Liu Y., Lee J.Y., Seo K., Hoh R.A., Pham T., Park J.H., Furman D., Dekker C.L., Davis M.M., James J.A., Nadeau K.C., Cunningham-Rundles C, & Boyd S.D. (2015). IgH sequences in common variable immune deficiency reveal altered B cell development and selection. Science translational medicine, 7(302), 302ra135.

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Tonsil CD4+ T cell isolation

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Tonsils were mechanically disrupted by first cutting the tissue samples with a scalpel into small pieces, and then dissociating into single cells using gentleMACS technology (C tube and gentleMACS; Miltenyi Biotec). Alternatively, cells were dissociated by forcing through a 40-µm cell strainer. Single-cell suspension was then enriched for light density cells by a Ficoll gradient centrifugation (Lymphoprep). Tonsil CD4⁺ T cells were enriched using CD4⁺ T cell isolation kit (Miltenyi Biotec) and used for intracellular flow cytometry or further purified by cell sorting. Naive CD4⁺ T cells, GC Tfh cells, and extra-follicular Tfh cells were sorted using antibodies against CD4 (clone RPA-T4; BioLegend), CD19 (clone HIB19; eBioscience), CD45RO (clone UCHL1; BD Biosciences), CXCR5 (clone RF8B2 from BD Biosciences or clone J252D4 from BioLegend), and PD-1 (clone EH12.1 or clone EH12.2H7 from BD Biosciences) on a FACS Aria instrument (BD Biosciences).

Durand M., Walter T., Pirnay T., Naessens T., Gueguen P., Goudot C., Lameiras S., Chang Q., Talaei N., Ornatsky O., Vassilevskaia T., Baulande S., Amigorena S, & Segura E. (2019). Human lymphoid organ cDC2 and macrophages play complementary roles in T follicular helper responses. The Journal of Experimental Medicine, 216(7), 1561-1581.

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Xenograft Cell Characterization Protocol

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Cells harvested from xenografts were analysed for the expression of human CD44 (clone DB105; Miltenyi Biotec), human CD24 (clone 32D12; Miltenyi Biotec), human EpCAM (clone CD326; Miltenyi Biotech), human B lymphocyte antigen, CD19 (clone HIB19; eBioscience), human neural cell adhesion molecule, CD56 (clone AF12-7H3; Miltenyi Biotec) and human CD45 (clones H130 and 2D1; eBioscience) following mouse cell depletion (Miltenyi Biotech).
All cells were analysed on a Cyan ADP flow cytometer (Dako Cytomation) and data processed using Summit v4.3 software (Beckman Coulter). Based on flow cytometric analysis we estimated >98% of cells were donor-derived.

Taurozzi A.J., Beekharry R., Wantoch M., Labarthe M.C., Walker H.F., Seed R.I., Simms M., Rodrigues G., Bradford J., van der Horst G., van der Pluijm G, & Collins A.T. (2017). Spontaneous development of Epstein-Barr Virus associated human lymphomas in a prostate cancer xenograft program. PLoS ONE, 12(11), e0188228.

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Multiparametric Flow Cytometry Analysis of Stimulated B Cells

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B cells were Fc blocked (BD Biosciences, San Jose, CA) prior to staining. Cells were analyzed using an LSR Fortessa cytometer (BD Biosciences), with gating performed by first setting a wide lymphocyte gate by forward and side scatter, followed by two sets of doublet exclusion, first by FSC-A by FSC-H and then by FSC-A by FSC-W, and by excluding dead cells with a viability stain (Life Technologies, Grand Island, NY). B cell purity was determined during isolation and at baseline by staining for CD19 (clone HIB19, eBiosciences, San Diego, CA). Cell phenotype was determined by staining IgM (clone MHM-88), CD27 (clone O323), and CD38 (clone HIT2, all from BioLegend, San Diego, CA), with results reported for the live cells gate. B cell proliferation was determined by dilution of CFSE dye, with results reported for the live singlet lymphocyte gate.

Simchoni N, & Cunningham-Rundles C. (2015). TLR7 and TLR9 responsive human B cells share phenotypic and genetic characteristics. Journal of immunology (Baltimore, Md. : 1950), 194(7), 3035-3044.

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Flow Cytometry Analysis of PBMCs and CSF

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Flow cytometry analysis was performed using antihuman antibodies to BDCA-2 (clone 201A; BioLegend, San Diego, CA), CD33 (clone P67.6; BioLegend), CD14 (clone 61D3; eBioscience, San Diego, CA), Lyve-1 (clone 537028; R&D Systems, Minneapolis, MN), CD3 (clone SK7; BioLegend), CD19 (clone HIB19; eBioscience), CD16 (clone B73.1; BioLegend), CD1c (clone L161; BioLegend), Lox-1 (clone 15C4; BioLegend), and human leukocyte antigen DR (HLA-DR) (clone Immu-357; Beckman Coulter, Brea, CA). One million PBMCs and between 2 × 10⁴ and 40 × 10⁴ CSF cells were incubated with human-Fc block (BD Biosciences, San Jose, CA) for 10 minutes at RT and then with antibodies for 20 minutes at 4°C. Subsequently, the samples were washed with PBS + 2% FBS (flow buffer) for 5′, spun at 500g, and resuspended in 200 μL of flow buffer. The samples were run on a Gallios flow cytometer (Beckman Coulter Life Sciences) on the same day of the collection. The cells were analyzed using FlowJo software (Tree Star, Ashland, OR).

Esaulova E., Cantoni C., Shchukina I., Zaitsev K., Bucelli R.C., Wu G.F., Artyomov M.N., Cross A.H, & Edelson B.T. (2020). Single-cell RNA-seq analysis of human CSF microglia and myeloid cells in neuroinflammation. Neurology® Neuroimmunology & Neuroinflammation, 7(4), e732.

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