100 m cpsil 88 fused capillary column

The fatty acid composition of the three experimental diets was measured by gas chromatography (GC). Briefly, total lipids from diets were extracted using the Folch method (23 (link)), saponified, and then methylated with hexane and BF3 (boron trifluoride). For the liver, a modified Folch method was used to extract total lipids and phospholipids as previously described (24 (link), 25 (link)). Fatty acid methyl esters were separated and identified by automated GLC (GLC7890A; Agilent Technologies) on a 100-m CPSIL 88 fused capillary column (100 m × 0.25 mm; Agilent) as described previously (26 (link)).

Total lipids were extracted with chloroform/methanol (2:1 vol/vol) using a modification of the Folch procedure [50 (link)]. Thin layer chromatography on silica G plates was used to isolate total phospholipids. Samples were scraped and methylated at 110 °C with a mixture (1:1) of BF3/methanol reagent (Sigma) and hexane. Fatty acid methyl esters were separated and identified by gas liquid chromatography (Agilent Model7890A, Agilent Technologies) using a 100 m CP-Sil 88 fused capillary column (100 m × 0.25 mm, Agilent Technologies, Santa Clara, CA, USA) and STD 502 (NuChek, Elysian, MN, USA) [51 (link)]. Fatty acids with a 14 to 24 carbon chain length were quantified and are presented as % of total fatty acids.

The fatty acid composition of the three experimental diets was measured by gas chromatography (GC). Briefly, total lipids from diets were extracted using the Folch method (23 (link)), saponified, and then methylated with hexane and BF3 (boron trifluoride). For the liver, a modified Folch method was used to extract total lipids and phospholipids as previously described (24 (link), 25 (link)). Fatty acid methyl esters were separated and identified by automated GLC (GLC7890A; Agilent Technologies) on a 100-m CPSIL 88 fused capillary column (100 m × 0.25 mm; Agilent) as described previously (26 (link)).