CUR was purchased from Shanghai D&B biological science and technology Co., Ltd. (Shanghai, China). PEG5k-b-PPLA5k was purchased from Jinan Daigang Biomaterial Co., Ltd. (Jinan, China). MDA-MB-231 cells were obtained from the ATCC (Manassas, VA, USA). MTT was supplied by Sigma (St. Louis, MO, USA). Rabbit anti-human anti-STAT3 (BM4052), anti-phospho-STAT3 (Tyr705) (BM4835), anti-JAK2 (BM4165), and anti-phospho-JAK2 (Tyr1007/Tyr1008) (BM4839) antibody products were obtained from Boster Biological Technology Co., Ltd. (Wuhan, China). HRP-labeled Goat Anti-Rabbit IgG (A0208) and the Annexin V-FITC apoptosis detection kit (C1062M) were purchased from Beyotime Biotechnology (Shanghai, China). 24-well transwell chambers (Cat. No. 3422) and polymerized Matrigel (Cat. No. 356234) were bought from Corning (Corning, NY, USA).
Hrp labeled goat anti rabbit igg a0208
Manufactured by Beyotime
Sourced in China
HRP-labeled Goat Anti-Rabbit IgG (A0208) is a secondary antibody labeled with Horseradish Peroxidase (HRP). It is designed to bind to rabbit primary antibodies and can be used for various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 231 cell, by American Type Culture Collection (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Annexin 5 fitc apoptosis detection kit c1062m, by Beyotime (1 mentions)
Ab32047, by Abcam (1 mentions)
Ab3760, by Abcam (1 mentions)
Anti p ampkα, by Cell Signaling Technology (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Anti cyclin d1, by Cell Signaling Technology (1 mentions)
4 protocols using hrp labeled goat anti rabbit igg a0208
1
Cell Migration and Apoptosis Assay Protocol
2
Immunohistochemistry of Cochlear AMPK
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Cochlear paraffin sections were deparaffinized with xylene and rehydrated in ethanol at graded concentrations. Rinsed in 3% hydrogen peroxide for 10 minutes and block solution for 30 minutes at room temperature. Then immersed in the following primary antibodies in a wet box at 4 °C overnight: anti-p-AMPKα (2535, Cell Signaling Technology Inc., Beverly, MA, USA) at 1:100, anti-AMPKα1 (ab32047, Abcam, Cambridge, England, UK) at 1:200 and anti-AMPKα2 (ab3760, Abcam, Cambridge, England, UK) at 1:100, respectively. Then incubated in the secondary antibodies HRP-labeled goat anti-rabbit IgG (A0208, Beyotime, Shanghai, CN) for 30 minutes in darkness at room temperature. The peroxidase reaction was visualized by using diaminobenzidine (DAB) reagent. The slides were finally dehydrated, cleared and mounted with coverslips.
3
Western Blot Protein Analysis
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Proteins were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Merck KGaA, Darmstadt, Germany). The membranes were blocked for 2 h at room temperature (RT) with 5% non-fat milk in Trisbuffered saline (TBS) plus 0.1% Tween-20 (TBST), which was followed by an overnight incubation at 4 °C with antibodies diluted in the blocking buffer. After three 10-min washes with TBST, blots were incubated at RT for 1 h with the appropriate secondary antibody conjugated to horseradish peroxidase, and protein expression was detected with an enhanced chemiluminescent reagent (Beijing 4A Biotech, Beijing, China). The following antibodies were used: monoclonal anti-GAPDH (AG019, 1:1000) and HRP-labeled Goat Anti-Rabbit IgG (A0208, 1:500) were from Beyotime Biotechnology (Nantong, China); polyclonal anti-AXSL1 (Catalogue #52519, 1:1000) and Anti-cyclin D1 (Catalogue #2922, 1:1000) were from Cell Signaling Technology (Massachusetts, USA).
4
Immunoblot Analysis of Proteins
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Total proteins extracted from the treated cells using extraction buffer (100 mM Tris-HCl pH 7.5, 50 mM NaCl, 5 mM EDTA, 1 mM PMSF, and 1 mM DTT) were separated using SDS-polyacrylamide gel electrophoresis (PAGE). Then the proteins were transferred to polyvinylidene fluoride (PVDF) membranes, blocked with 5% milk at room temperature for 2 h, and incubated overnight at 4 °C with the antibodies. Immunoreactive bands were visualized using chemiluminescence after binding to the corresponding secondary antibody. The secondary antibodies were horseradish peroxidase (HRP)-labeled goat anti-mouse IgG (A0216, Beyotime, Shanghai, China) and the HRP labeled goat anti-rabbit IgG (A0208, Beyotime, Shanghai, China). Immunoreactivity was determined using the ECL method (K-12045-D50, Advansta, California, USA), according to the manufacturer's instructions (Zhou et al., 2021 (link)).
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