PANC-1 cells were harvested after treatment, washed, and lysed with RIPA lysis buffer containing protease inhibitors (Hoffman-La Roche Ltd., Basel, Switzerland). Protein concentration was determined using the Bradford protein assay. Protein extracts were separated using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Membranes were blocked and incubated with primary Bax (mouse monoclonal, clone 2D2, 1:500; Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), Bcl-xL (rabbit polyclonal, 1:500; Cell Signaling Technology Inc., Danvers, MA, USA), caspase-3 (mouse monoclonal, clone 3G2, 1:500; Cell Signaling Technology Inc.), caspase-8 (mouse monoclonal, clone 1C12, 1:500; Cell Signaling Technology Inc.), cPARP (rabbit anti cPARP, 1:700; Cell Signaling) heat shock protein 70 (HSP70) (mouse anti-HSP70, 1: 1,200; StressMarq Biosciences Inc.), or β-actin-HRP antibody (mouse monoclonal, clone 8226, 1:25,000; Abcam, Cambridge, UK) before incubation with secondary anti-mouse IgG-HRP antibody or anti-rabbit IgG-HRP antibody (1:25,000; both from Santa Cruz Biotechnology Inc., Dallas, TX, USA). Immunosignals were detected using chemoluminescence HRP substrate (EMD Millipore, Billerica, MA, USA).
Anti mouse igg hrp antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom, United States
The Anti-mouse IgG-HRP antibody is a secondary antibody that binds to mouse immunoglobulin G (IgG) and is conjugated with horseradish peroxidase (HRP). This antibody can be used for the detection and quantification of mouse IgG in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
C parp, by Cell Signaling Technology (1 mentions)
Caspase 8, by Cell Signaling Technology (1 mentions)
Anti rabbit igg hrp antibody, by Santa Cruz Biotechnology (1 mentions)
Protease inhibitor, by Roche (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Bcl2 associated x (bax), by Santa Cruz Biotechnology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
Ai600 imaging system, by GE Healthcare (1 mentions)
Trans blot turbo system, by Bio-Rad (1 mentions)
Caspase 3 and caspase 9 colorimetric assay kits, by R&D Systems (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (1 mentions)
Growth supplement, by Thermo Fisher Scientific (1 mentions)
Anti bcl 2 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti p53 antibody, by Santa Cruz Biotechnology (1 mentions)
Anti bax antibody, by Santa Cruz Biotechnology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Culture media, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti mouse igg hrp antibody
1
Protein Expression Analysis in PANC-1 Cells
2
TeNT Cleavage Assay of Synaptic Proteins
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Recombinant TeNT light chain protein (R&D Systems, 6535-ZN) was dissolved in recording internal at various concentrations along with recombinant GFP/SNAP25B/VAMP-2 (R&D Systems, 7375-SV). Quality control and purification specifications were provided by R&D Systems (we did not perform additional verification of these data). Samples were incubated at 31 °C for 1 h, after which 10 µl of TeNT cleaved and control samples were resolved with a 10% SDS-PAGE gel (Bio-Rad, 1610738) in Tris/SDS/glycine buffer (Bio-Rad, 1610732) at 150 V for 2 h and then transferred to PVDF membranes using a Trans-Blot Turbo System (Bio-Rad, 1704150EDU). PVDF membranes were blocked with 5% non-fat milk/TBS and incubated with anti-GFP antibody (1:1,000, Santa Cruz Biotechnology, sc-9996) and anti-mouse IgG HRP antibody (1:5,000, Santa Cruz Biotechnology, SC-516102-CM). Chemiluminescent signals were generated by an ECL kit (Pierce, 32109) and recorded with a AI600 imaging system (GE Healthcare).
3
Apoptosis Induction and Oxidative Stress Assessment
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