Antibodies to p stat3

Antibodies to p‐STAT3 (#9145), STAT3 (#4904), GAPDH (#5174) were purchased from Cell Signaling Technology (Danvers, USA). Antibodies to Cytokeratin (#10830‐1‐AP), Vimentin (#10366), Collagen I (#14695), α‐SMA (#14395) and CD45 (#20103‐1‐AP) were purchased from Proteintech (Rosemont, USA). Anti‐Factor VIII (#NB100‐91761) was from Novus Biologicals (Centennial, USA). Dispase II was purchased from Roche (Mannheim, Germany). Recombinant rat IL‐6 protein (#506‐RL), mouse IL‐6R protein (#1830‐SR), rat gp130 Fc protein (#5029‐RG) and mouse TGF‐beta 1 protein (#7666‐MB) were purchased from R&D Systems (Minneapolis, USA). The STAT3‐specific inhibitor S3I‐201 (#SML0330) and CG (#C9394) were purchased from Sigma‐Aldrich (Darmstadt, Germany). For in vivo studies, S3I‐201 was further diluted with the medium and the concentration of DMSO was below 0.001%. For in vitro studies, S3I‐201 was further diluted with PBS and the concentration of DMSO was below 0.01%. DMEM/F12 and other cell culture reagents were obtained from Gibco Corp (Carlsbad, USA).

Antibodies to pSTAT3 (tyrosine 705), pJAK3, JAK3, and PTPN6 were purchased from Cell Signaling Technologies (Beverly, MA, USA) Antibody to β-Actin was purchased from Santa Cruz (Dallas, TX, USA). P-tyrosine (4G10) antibody was purchased from Millipore. The pharmacological inhibitors romidepsin and azacytidine were purchased from Sigma-Aldrich. JAK3 inhibitor WHIP-154 was purchased from Santa Cruz Biotechnology and JAK2 inhibitor ruxolitinib was procured from ChemieTek (Indianapolis, IN).