Drp 220at

Commercial screen-printed electrodes (SPE, Model No: DRP 220 AT, Φ = 4 mm), including a Au working electrode (WE) with a surface area of 12.56 mm², were purchased from the Metrohm DropSens Co. (Oviedo, Spain). This electrode includes a Au counter electrode (CE) and a silver pseudo-reference electrode (RE). Chronoamperometry (CA) and cyclic voltammetry (CV) measurements were conducted with a multi-channel potentiostat obtained from CH instruments (Texas, TX, USA, Model No: CH 1030C) and Wismar Co. Ltd. (Daejeon, Korea, Model No: WIZECM-1200Premium). Electrochemical impedance spectroscopy (EIS) was carried out with a pocketSTAT from Ivium Technologies B.V. (AJ Eindhoven, The Netherlands). All electrochemical (EC) measurements were conducted at room temperature in a Faraday cage.

Commercial SPE (Model no: DRP 220 AT, Φ = 4 mm) was purchased from Metrohm DropSens (Asturias, Spain). CA, and CV, EIS were performed with a potentiostat obtained from CH Instruments (Texas, USA; Model no: CH 660C). All electrochemical measurements were performed at room temperature in a Faraday cage to ensure electromagnetic shielding.

Unless otherwise stated, chemicals and reagents were used without further purification and were of analytical grade. Screen-printed gold electrodes (SPE-Au) with a three-electrode system (DRP-220AT) were purchased from Metrohm Dropsens (Spain). In the three-electrode system, working (4 mm diameter), reference and counter electrodes consisted of gold, silver/silver chloride, and platinum, respectively. UltraPure^TM DNase/RNase-free distilled water was purchased from Invitrogen, Australia. Synthetic lncRNA and capture probes (Table 1) were purchased from Integrated DNA Technologies (Coralville, IA, USA). All other reagents were purchased from Sigma Aldrich (Sydney, NSW, Australia). All electrochemical measurements were performed using a 650E series electrochemical workstation potentiostat purchased from CH Instruments, Austin, TX, USA. Plasma samples (two benign and four ovarian carcinomas) were obtained from UQCCR according to the declaration of Helsinki, with approval from the Ethics Committee of the University of Queensland (approval number 2016000300) and the Ochsner Medical centre (New Orleans, LA, USA). MeT-5A, SKOV3, and OVCAR3 cell lines were cultured in the laboratory.