Human tongue cancer cell line (CAL 27) was provided by Procell Life Science & Technology (Wuhan, China) and was cultured in DMEM supplemented with 1 penicillin/streptomycin and 20% FBS at 37°C under one atmosphere with 5% CO2 in humidified air. Curcumin was produced by Solarbio Life Sciences Inc. (Beijing, China).
Cal 27
Manufactured by Procell
Sourced in China
The CAL-27 is a laboratory equipment used for cell culture applications. It is a cell line derived from human tongue squamous cell carcinoma. The core function of the CAL-27 is to provide a standardized cell line for research and experimentation purposes.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Scc 25, by Procell (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Tca8113, by Procell (3 mentions)
Cal27, by Thermo Fisher Scientific (3 mentions)
Tscca, by Procell (2 mentions)
Dmem f12, by Thermo Fisher Scientific (2 mentions)
Dmem medium, by Thermo Fisher Scientific (2 mentions)
Tca8113, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (2 mentions)
Curcumin, by Solarbio (1 mentions)
Rmpi 1640, by Thermo Fisher Scientific (1 mentions)
Tscca, by Thermo Fisher Scientific (1 mentions)
Scc25, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Clark Bioscience (1 mentions)
Mem medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Dulbecco modified eagle medium (dmem), by Boster Bio (1 mentions)
Penicillin streptomycin solution, by Solarbio (1 mentions)
16 protocols using cal 27
1
Culturing Human Tongue Cancer Cells
2
Culturing OSCC Cell Line Cal27
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OSCC cell line Cal27 was obtained from Procell and maintained in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin_streptomycin at 37 °C under 5% CO2. The cells were subcultured using 0.25% trypsin at 80–90% confluency, and the medium was changed every 2 days.
3
Transfecting TSCC Cell Lines with miR-532-3p
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The four TSCC cell lines TSCCA, TCA8113, CAL-27, and SCC-25 were obtained from Procell Life Science & Technology (Wuhan, China). TSCCA and TCA8113 cells were cultured in RMPI 1640 (Gibco, USA) plus 10% fetal bovine serum (FBS, Hyclone, USA). CAL-27 and SCC-25 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco, USA) plus 10% FBS and DMEM/F12 (Gibco, USA) plus 10% FBS, respectively. All cell lines were incubated in a cell incubator at 37°C in 5% CO2.
MiR-532-3p mimics/NC mimics and miR-532-3p inhibitor/NC inhibitor were transfected in CAL-27 and TCA8113 using Lipofectamine 2000 (Invitrogen, USA), respectively. MiR-532-3p mimics and CCR7/vector were co-transfected in CAL-27 using Lipofectamine 2000. The co-transfected cells were cultured in complete medium (DMEM+10% FBS+350 ng/ml CCL21). In addition, the CAL-27 cells were divided into two groups, Control and CCL21 (medium contains 350 ng/ml CCL21).
MiR-532-3p mimics/NC mimics and miR-532-3p inhibitor/NC inhibitor were transfected in CAL-27 and TCA8113 using Lipofectamine 2000 (Invitrogen, USA), respectively. MiR-532-3p mimics and CCR7/vector were co-transfected in CAL-27 using Lipofectamine 2000. The co-transfected cells were cultured in complete medium (DMEM+10% FBS+350 ng/ml CCL21). In addition, the CAL-27 cells were divided into two groups, Control and CCL21 (medium contains 350 ng/ml CCL21).
4
Human HNSC Cell Line Cultivation
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Human HNSC cell lines (FaDu and Cal27) were purchased from Procell Life Science and Technology Co. Ltd. (Wuhan, China). FaDu cells were cultured in MEM medium (Gibco, USA), and Cal27 cells were cultured in DMEM medium (Gibco, USA). All cultures were supplemented with 1% penicillin-streptomycin (Solarbio, China) and 10% fetal bovine serum (Clark, USA). All cells were cultured in incubators at 37 °C and 5% CO2.
5
Human Oral Squamous Cell Carcinoma Cell Lines
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The human OSCC cell lines CAL-27 and SCC-25 were purchased from Procell Life Science and Technology Co., Ltd. (Hubei, China). The HOK cell line was provided by the School and Hospital of Stomatology at Shanxi Medical University. The CAL-27 cell line was grown in Dulbecco’s Modified Eagle Medium (DMEM, Boster, China), the SCC-25 cell line was grown in DMEM/Nutrient Mixture F-12 (DMEM/F12, Boster, China), and the HOKs were grown in Hyclone1640 medium (Hyclone, USA). The medium contained 10% fetal bovine serum (Gibco, USA) and 1% penicillin-streptomycin solution (Solarbio, China) and was maintained in an incubator with 5% CO2 at 37 °C.
6
Oral Cancer Cell Culture Protocol
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Oral epithelial cells (HOEC) and OSCC cell line Cal27 were purchase from Procell (Wuhan, China), OSCC cell lines (SCC-4, SCC-15, SCC-9, SCC-25, and Tca83) were from ATCC (Manassas, VA). All cells were hatched in DMEM (Gibco) with L-glutamine, sodium pyruvate, 10% fetal bovine serum (FBS, Sigma) at 37 °C and 5% CO2.
7
Cell Culture Conditions for HEK-293T, Cal27, and UM1
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The SV40-transformed human embryonic kidney cell line HEK-293T, as well as HNSCC cell line Cal27, were purchased from Procell and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Biology industry, Shanghai, China) and 1% penicillin and streptomycin (Biosharp, Shanghai, China). The human head and neck squamous cell line UM1 was a generous gift from Prof. Anxun Wang (Department of Oral and Maxillofacial Surgery, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China) [75 (link),76 (link),77 (link),78 (link)], and cultured in DMEM/F12 medium (Hyclone, UT, USA) supplemented with 5% FBS and 1% penicillin and streptomycin. Cell culture was carried out at 37 °C, 5% CO2 and 95% humidity.
8
Culturing and Transfecting Oral Cancer Cell Lines
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The human normal oral epithelial cell (HOEC) line was purchased from BeNa Culture Collection (BNCC, Beijing, China) and cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco, GrandIsland, NY, USA) supplemented with 10% foetal bovine serum (Hyclone, USA). The human TSCC cell lines CAL-27 and SCC-25 cell lines, both of which were purchased from Procell Life Science & Technology Co., Ltd (Wuhan, China), were kept in Gibco-provided DMEM and Gibco-provided DMEM/F12, respectively. Another TSCC cell line (CTSC-3) was obtained from ChemicalBook (Shanghai, China) and cultured in Roswell Park Memorial Institute (RMPI) 1640 Medium (Gibco). All the cell lines were cultured in their respective medium at 37°C in an atmosphere containing 5% CO2. Cell transfection was conducted when the cell grown to 50% confluence using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, San Jose, CA, USA). Small interfering RNA targeting PDPN (si-PDPN), the miR-532-3p mimic, the miR-532-3p inhibitor, and the negative control (NC) were purchased from RiboBio (Guangzhou, China), and were transfected at a concentration of 50 nmol/L. The transfected cells were collected after 48 h for further analyses.
9
Promoter Variants Regulate NFKB1 Expression
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About −800 to 60 region of the NFKB1 promoter containing −94 Ins allele (rs28362491) and −449 C allele (rs72696119) were synthesized and constructed into the pGL3‐Basic reporter plasmid (Promega, Madison, WI) named PGL3‐NFKB1‐IC. Using site‐directed mutagenesis technique, PGL3‐NFKB1‐IC was then mutated to PGL3‐NFKB1‐DC, PGL3‐NFKB1‐IG, and PGL3‐NFKB1‐DG, respectively. Sequence analysis was performed to confirm these plasmids. Subsequently, human tongue squamous cell carcinoma cell lines Tca‐8113 (Procell, Wuhan, China) and CAL‐27 (Procell) in 12‐well plates were transfected with reporter plasmids (1.0 μg) and plasmid pRL‐TK (1.0 μg) (Promega) as an internal control containing renilla luciferase reporter gene. After 12 h, luciferase activities were detected using a dual‐luciferase assay kit (Beyotime, Beijing, China) following the manufacturer's instructions. Relative luciferase activity was calculated according to the RLU (relative light unit) of the firefly luciferase divided by the RLU of the renilla luciferase.
10
Culturing Oral Cell Lines
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Normal oral epithelial cells (CP-H203), human oral mucosal fibroblasts (CP-H205, named normal fibroblasts, NFs thereafter), and OCSCC cell lines SCC-25 (CL-0569) and CAL-27 (CL-0265) were purchased from Procell (Wuhan, Hubei, China) and cultured using DMEM containing 10% FBS with 1% penicillin/streptomycin at 37 °C with 5% CO2.
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