Chemi doc documentation system quantity one quantitation software

Manufactured by Bio-Rad

Sourced in United States

The Chemi Doc Documentation System is a versatile imaging platform designed for the detection and quantitation of a variety of samples. The system features a camera, a dark enclosure, and software for image capture and analysis. The Quantity One software provides tools for quantitative analysis of the captured images.

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Lab products found in correlation

Protease inhibitor cocktail, by Merck Group (2 mentions) Immobilon p, by Merck Group (1 mentions) Anti mouse igg peroxidase conjugate, by Merck Group (1 mentions) Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions) Hyperfilm ecl, by GE Healthcare (1 mentions) Immobilon p membrane, by Merck Group (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Quantity One Quantitation Software Quantity One software Quantity One System Quantitation One software Quantity One

3 protocols using chemi doc documentation system quantity one quantitation software

Western Blot Analysis of Neural Stem Cell Markers

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Cells from both monolayer cultures and NS, after 0–45 days of culture, were collected in a lysis buffer (150 mM NaCl, 1% Nonidet P-40, 0.5% Triton X-100, 0.5 mM EDTA, 0.1% SDS, 50 mM Tris-HCl, pH 7.6), containing protease inhibitor cocktail (Sigma-Aldrich), and 17.4 µg/ml phenylmethylsulfonyl fluoride (Sigma-Aldrich). Protein extracts were quantified by Bradford Protein Assay. For Western-blot analysis, 50 µg of total proteins were resolved on denaturating polyacrylamide gels (SDS-PAGE): 10% (Msi-1, βIII-Tubulin) and 8% (Nestin) and transferred to polyvinylidene difluoride (PVDF; Immobilon P, Millipore, USA) membrane by electroblotting. Membranes were blocked with PBS-T (PBS buffer saline with 0.1% Tween-20) containing 5% BSA and then incubated overnight with primary antibodies. After washing for 3×5 min., membranes were probed 1 h at room temperature with secondary antibody (anti-mouse IgG peroxidase conjugate, Sigma, 1∶10,000). The revelation was performed by chemiluminescence.
The signals were quantitated by densitometric scanning (ChemiDoc documentation system/QuantityOne quantitation software; Bio-Rad Laboratories, Hercules, CA, USA).

Iacopino F., Angelucci C., Piacentini R., Biamonte F., Mangiola A., Maira G., Grassi C, & Sica G. (2014). Isolation of Cancer Stem Cells from Three Human Glioblastoma Cell Lines: Characterization of Two Selected Clones. PLoS ONE, 9(8), e105166.

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SCD1 and SCD5 Protein Quantification in Tumor Cells

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For SCD1 and SCD5 analysis, tumor cell total protein extracts were obtained in RIPA lysis buffer [50 mM Tris-HCl (pH 7.7), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS] freshly supplemented with phosphatase and protease inhibitors (100 μM Na₃VO₄, 0.3 mM phenylmethylsulfonylfluoride, 50 μg/ml leupeptin and 20 μg/ml aprotinin). Thirty μg of total proteins were resolved on a 10% SDS-PAGE and transferred onto an Immobilon P membrane (Millipore, Billerica, MA, USA) which was incubated with the primary antibodies. The blots were then overlaid with a HRP-labelled secondary antibody. The protein bands were detected using an enhanced chemiluminescence system (ECL, GE Healthcare, Piscataway, NJ, USA) and visualised on Hyperfilm ECL (GE Healthcare). β-actin was used as an internal loading control. Band intensities were measured by densitometry (Chemi Doc Documentation System/Quantity One quantitation software, Bio-Rad). Variations in SCD1 and SCD5 protein levels were expressed as fold change compared to control (MCF-7 or MDA-MB-231 cells cultured alone) after normalization to β-actin expression.

Angelucci C., D'Alessio A., Iacopino F., Proietti G., Di Leone A., Masetti R, & Sica G. (2018). Pivotal role of human stearoyl-CoA desaturases (SCD1 and 5) in breast cancer progression: oleic acid-based effect of SCD1 on cell migration and a novel pro-cell survival role for SCD5. Oncotarget, 9(36), 24364-24380.

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Western Blot Protein Quantification

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Briefly, cells were lysed with RIPA lysis buffer in the presence of protease inhibitor cocktail (Merck, Darmstadt, Germany). Cell debris was removed by centrifugation at 12, 000 rpm for 12 min. The protein content was determined with BCA kit and separated by 8~10% SDS-PAGE, and then transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA). The membranes were blocked in 5% (w/v) non-fat dry milk in Tris-buffered saline (TBS) and 0.1% (v/v) Tween 20 for 1 h at room temperature, and then incubated with the primary antibodies (1:500; Abcam, Cambridge, MA, USA) at 4 °C overnight. GAPDH (1:1000; Santa Cruz Biotechnology, Paso Robles, CA, USA) was used as an intern control. After incubation of appropriate secondary horseradish peroxidase-conjugated antibodies including HRPconjugated anti-rabbit or anti-mouse (1:1000; Cell Signaling Technology, Inc., Beverly, MA, USA) for 1 h, blots were visualized with ECL kit. Band intensities were measured by densitometry (Chemi Doc Documentation System/Quantity One quantitation software, Bio-Rad Laboratories Inc, Hercules, CA, USA). Variations in protein levels were expressed as fold change compared with control after normalization to GAPDH.

Yao Y., Jia H., Wang G., Ma Y., Sun W, & Li P. (2019). miR-297 Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Inflammatory Response and Apoptosis. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 52(4).

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