1 ethyl 3 3 dimethylaminopropyl carbodiimide edc

Manufactured by Fluorochem

Sourced in United Kingdom

1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) is a water-soluble carbodiimide. EDC is commonly used as a coupling agent in biochemical and organic chemistry applications.

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1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (2 citations)

3 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide edc

Diverse Reagent Preparation for Biomolecular Analysis

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Xylene, ethanol, trifluoroacetic acid (TFA), and ammonium hydroxide 25% were purchased from Merck (Darmstadt, Germany). Dimethylamine 40% (DMA), 1-Hydroxybenzotriazole hydrate (HOBt), Mayer’s hematoxylin solution, and eosin Y were from Sigma-Aldrich (Taufkirchen, Germany). Anhydrous dimethyl sulfoxide (DMSO) was from Applichem (Darmstadt, Germany). The 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was from Fluorochem (Hadfield, UK). Acetonitrile (ACN) was from VWR Chemicals (Darmstadt, Germany). Citric acid was from Carl Roth (Karlsruhe, Germany). The α-2,4-hydroxycinammic acid (CHCA) was from Bruker Daltonics (Bremen, Germany). PNGase F PRIME was from N-Zyme Scientifics (Doylestown, PA, USA). Dextran hydrolysate (DH) was prepared in-house from dextran (Oxford, UK). Milli-Q grade water was generated in-house using a Water purification system, MilliQ Plus (Merck Millipore, Darmstadt, Germany).

Grzeski M., Taube E.T., Braicu E.I., Sehouli J., Blanchard V, & Klein O. (2022). In Situ N-Glycosylation Signatures of Epithelial Ovarian Cancer Tissue as Defined by MALDI Mass Spectrometry Imaging. Cancers, 14(4), 1021.

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Mass Spectrometry-Based Glycoprotein Analysis

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Sodium bicarbonate, glacial acetic acid (HAc), hydrogen peroxide and ethanol (EtOH) were obtained from Merck (Darmstadt, Germany). α-cyano-4-hydroxycinnamic acid (CHCA), 2-picoline borane, dimethylsulfoxide (DMSO), 1-hydroxybenzotriazole hydrate (HOBt), 50% sodium hydroxide, ammonium acetate, and trifluoroacetic acid (TFA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). HPLC SupraGradient acetonitrile (ACN) was obtained from Biosolve (Valkenswaard, The Netherlands). 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and hydrochloride acid was obtained from Fluorochem (Hadfield, UK). Recombinant PNGase F PRIME-LY™ Glycosidase from Flavobacterium meningosepticum was purchase from N-Zyme Scientifics (Doylestown, PA, USA). Peptide calibration standard II was purchased from Bruker Daltonics (Bremen, Germany). All buffers were prepared using deionized water (mQ) generated from a Q-card 2 system (Millipore, The Netherlands). Poly-horseradish peroxidase solution (Poly-HRP) was obtained from Immunologic (Duiven, The Netherlands). 3,3’-diaminobenzidine (DAB) was obtained from DAKO (Agilent Technologies, Santa Clara, CA, USA).

Boyaval F., Dalebout H., Van Zeijl R., Wang W., Fariña-Sarasqueta A., Lageveen-Kammeijer G.S., Boonstra J.J., McDonnell L.A., Wuhrer M., Morreau H, & Heijs B. (2022). High-Mannose N-Glycans as Malignant Progression Markers in Early-Stage Colorectal Cancer. Cancers, 14(6), 1552.

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Synthesis and Characterization of Polymeric Compounds

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N-Vinylformamide (NVF) and N-vinylacetamide (NVA) were purchased from TCI. Methyl pentafluorobenzoate was purchased from abcr. Sodium azide was purchased from Roth. Magnesium sulfate, triethylamine, and N-hydroxysuccinimide (NHS) were purchased from Sigma Aldrich. 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) was purchased from Fluorochem. Rhodamine-B-isothiocyanate was purchased from ChemCruz. All solvents, HCl, and NaOH were purchased from VWR. All chemicals were used as received except NVF, which was distilled before usage. Poly(1-methylpenten) (TPX) and poly(vinylidene fluoride) (PVDF) were purchased from Goodfellow. PTFE foil was purchased from RCT. PVDF and PTFE were washed with CHCl 3 , EtOH and water while TPX was washed with EtOH and water before further modification.

Fischer T., Tenbusch J., Möller M, & Singh S. (2022). A facile method for grafting functional hydrogel films on PTFE, PVDF, and TPX polymers. Soft matter, 18(22).

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