Cells were homogenised in lysis buffer [10-mM Tris-HCl (pH 7.4), 150-mM NaCl, 1% NP-40, 10-mM NaF, 0.2-mM Na3VO4 and protease inhibitor (Sigma–Aldrich)]. Proteins (30 μg) were fractionated on 10% SDS-PAGE, blotted onto a polyvinylidene difluoride membrane (Millipore, MA) and probed with primary antibodies, including SRF, LAMC1 (Santa Cruz, CA) and Apaf-1 (Proteintech, Wuhan). GAPDH (ZSGQ-BIO, Beijing) was used as a loading control. The secondary antibodies were HRP-conjugated anti-rabbit (NA 934V) and anti-mouse (NA 931V) antibodies (GE Healthcare Life Sciences, MA). Proteins were visualised by chemiluminescence according to the manufacturer's instructions (Thermo, PA), followed by exposure to X-ray film. The density of bands was densito-metrically quantified using ImageJ (National Institutes of Health, MD).
Hrp conjugated anti rabbit na 934v
Manufactured by GE Healthcare
The HRP-conjugated anti-rabbit (NA 934V) is a laboratory reagent used in various immunological techniques. It consists of anti-rabbit antibodies that are covalently linked to horseradish peroxidase (HRP), an enzyme commonly used as a reporter molecule in these applications.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
Lamc1, by Santa Cruz Biotechnology (1 mentions)
Apaf 1, by Proteintech (1 mentions)
Anti mouse na931v, by GE Healthcare (1 mentions)
Protease inhibitor, by Merck Group (1 mentions)
Anti v5 r960 25, by Thermo Fisher Scientific (1 mentions)
Anti cbfb, by Santa Cruz Biotechnology (1 mentions)
Anti his sc 8036, by Santa Cruz Biotechnology (1 mentions)
Anti ha, by BioLegend (1 mentions)
2 protocols using hrp conjugated anti rabbit na 934v
1
Western Blot Analysis of Protein Targets
2
Mapping HIV-1 Host Protein Interactions
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The antibodies used in this study are as follows: β-tubulin monoclonal antibody (NMS-410P; Covance), Anti-CUL5 (sc-13014; Santa Cruz Biotechnology), anti-EloB (sc-1144; Santa Cruz Biotechnology), anti-CBFb (sc-166142; Santa Cruz Biotechnology), anti-Vif (GTX80393; GeneTex), anti-USP8 (A7031; ABclonal), anti-HA (901513; Biolegend), anti-Myc (AHO0052; Invitrogen), anti-V5 (R960-25; Invitrogen), anti-SAMHD1 (TA502024; OriGene), anti-HLTF (14786-1-AP; Proteintech), anti-BST2 (13560-1-AP; Proteintech), anti-A3G (D221663;Sangon Biotech), anti-USP7 (26948-1-AP; Proteintech), anti-USP33 (20445-1-AP; Proteintech), anti-USP37 (18465-1-AP; Proteintech), anti-His (sc-8036; Santa Cruz Biotechnology). CAp24 mAb (1513) was purchased from the NIH AIDS Reagents Program. Secondary antibodies were alkaline phosphatase-conjugated anti-rabbit (115–055–045; Jackson ImmunoResearch) and anti-mouse (115–055–062; Jackson ImmunoResearch), HRP-conjugated anti-rabbit (NA934V; GE) and anti-mouse (sc-2005; Santa Cruz Biotechnology).
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