To evaluate the plasma membrane damage and the cell viability at 24 hrs, a colorimetric assay for measuring lactate dehydrogenase (LDH) was performed on ARPE-19 cell culture medium samples. The medium was tested using the LDH substrate included in a commercial kit (cytoxicity detection kit, Roche, Molecular Biochemicals, Mannheim, Germany). The absorbance values were measured at 450 nm using a microplate reader (Synergy HT Multi-Mode, Bio-Tek), and results were expressed as percentages of control (100%).
Cytoxicity detection kit
Manufactured by Roche
Sourced in Switzerland
The Cytoxicity Detection Kit is a laboratory equipment product designed to measure cell viability and cytotoxicity. It provides a quantitative assessment of cell death or cell lysis by detecting the release of lactate dehydrogenase (LDH) from damaged cells.
Automatically generated - may contain errors
4 protocols using cytoxicity detection kit
1
Evaluating Cell Membrane Integrity
2
Cytotoxicity Assay for Cell Viability
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Briefly, cells (1 × 104) were seeded into 96-well plates and cultured for 12 h. After being collected, the LDH release activity from damaged cells was measured using a Cytoxicity Detection Kit (Roche, Basel, Switzerland). The absorbance values at 490 nm were detected using a microplate reader (Bio-Rad).
3
Quantification of Cellular Biomarkers in PC12 Cells
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The concentration of CORT, LDH, SOD, GPx, and ATP in the PC12 cells were assessed using commercially available ELISA kit (USCN Business Co., Ltd, Wuhan, China), Cytoxicity Detection Kit (Roche, Basel, Switzerland), SOD kit (Nanjing Jiancheng Bioengineering Institute), GPx assay kit, and ATP Assay Kit, respectively, according to the manufacturer's instructions.
4
Quantifying Cell Death via LDH Release
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The LDH released from the cytosol of damaged cells was determined to assess cell death status. Briefly, cells were seeded into 96-well plates at 1 × 10 4 cells per well and cultured for overnight. After collected by centrifugation, the LDH release activity from damaged cells was measured using a Cytoxicity Detection Kit (Roche, Basel, Switzerland). The absorbance values at 490 nm were detected using a microplate reader (Bio-Rad). The LDH release in experimental groups was expressed as a percentage of the mean absorbance measured in control group.
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