Frozen mouse liver and placenta tissues were sliced into 10-µm sections, stained with Oil-red O (GenMed Scientifics, USA). Sections were photographed under an optical microscope (Nikon, Canada) and stained area was assessed by use of NIS-Elements BR 3.2 software.
Oil red o
Manufactured by Genmed Scientifics
Sourced in United States
Oil-red O is a fat-soluble dye used in histology and cell biology to stain neutral lipids and triglycerides. It is a commonly used staining technique for the detection and visualization of lipid-containing compounds in tissue sections and cell preparations.
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2 protocols using oil red o
1
Quantifying Lipid Content in Frozen Tissues
2
Maternal Lipid Metabolism and Fetal Development
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The mice were anesthetized with 10% chloral hydrate (3 ml/kg, Sigma) on the 18th day of pregnancy. The blood samples that were collected from the retro-orbital plexus were centrifuged. The serum FFA concentration was measured by a blood lipid chemical analysis kit (Wako Chemicals, Japan), and the TG, TC, HDL, and low-density lipoprotein (LDL) levels were measured using an automatic biochemical analyzer (Olympus, Japan). The fetuses and placental tissues were collected and weighed. The total number of fetus, the number of live fetuses, and the number of the absorbed fetuses were recorded. The liver and placental tissues were collected and fixed with formalin for 48 h. Following embedding and slicing (5 μm), conventional hematoxylin and eosin (H and E) staining was carried out. The liver and placental tissues of mice were frozen and cut into 10-μm sections and finally stained with Oil Red O (GenMed Scientifics, USA). The sections were photographed by an optical microscope (Nikon, Japan).
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