Confocal images were acquired using a Nikon Eclipse Ti 2 inverted A1 Confocal with a 100x TIRF objective (NA 1.45). For XZ projections, images were captured at Nyquist sampling and reconstructed in FIJI. Images were processed in FIJI (Schindelin et al., 2012 (link)) and Adobe Photoshop. Figures were made in Adobe Illustrator. A minimum of three biological replicates were performed for each experiment.
Eclipse ti 2 inverted a1 confocal
Manufactured by Nikon
The Eclipse Ti 2 inverted A1 Confocal is a high-performance microscope system designed for advanced imaging and analysis. It features a confocal optical system that enables high-resolution, three-dimensional imaging of samples. The Eclipse Ti 2 is optimized for a variety of applications, including live-cell imaging, protein localization studies, and more.
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Lab products found in correlation
2 protocols using eclipse ti 2 inverted a1 confocal
1
Confocal Imaging of Biological Samples
2
Imaging Protocols for Live-Cell Microscopy
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Unless otherwise noted, all images were acquired using a Nikon Eclipse Ti2 inverted A1 confocal microscope with a 63× oil objective and a z-step size of 0.5 µm. Time-lapse imaging for Videos 1 , 2 , and 3 was performed using the same Nikon Eclipse Ti2 inverted A1 confocal, but with a 40× oil objective equipped with a humidified chamber and temperature-controlled stage. Widefield images (noted in figure legends) were acquired with an inverted Zeiss Axiovert 200M microscope using a 63× oil objective, QE charge-coupled device camera (Sensicam), and Slidebook v. 6.0 software (Intelligent Imaging Innovations). Time-lapse imaging for Video 4 , Video 5 , Video 6 , and Video 7 was performed on the same Zeiss Widefield, with a 63× oil objective and a temperature-controlled stage. Images were processed in Fiji or 3i software.
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