Nod scid gamma mice nsg

Manufactured by Charles River Laboratories

Sourced in United Kingdom

NOD scid gamma mice (NSG) are an immunodeficient mouse model developed by Charles River Laboratories. The core function of NSG mice is to serve as a platform for engraftment of human cells and tissues.

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Lab products found in correlation

Matrigel basement membrane matrix, by Corning (1 mentions)

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NOD/SCID mice (365 citations) SCID mice (163 citations) NSG mice (74 citations) NOD/SCID (51 citations) NOD scid gamma (NSG) mice (23 citations) NSG (9 citations) NOD mice (8 citations) NOD/SCID/gamma chain-/-(NSG) mice (2 citations) NOD.Cg-PrkdcSCIDIl2rgtm1Wjl/SzJ (NOD-SCID gamma; NSG) mice (2 citations)

3 protocols using nod scid gamma mice nsg

NSG Mice Xenograft Tumor Model

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Young adult (5–6
weeks old) female NOD scid gamma mice (NSG; genotype:
NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/SzJ (005557)) were obtained from Charles River (UK) and housed for 2
days before tumor initiation. 3E.Δ.NT cells were trypsinized,
washed with prewarmed HBSS (without Ca²⁺ and Mg²⁺), resuspended, counted, and aliquots of 5 × 10⁵ cells
in 50 μL of HBSS were injected s/c into the left mammary fat
pad between nipples four and five. Once palpable, tumor volumes were
measured with calipers using the following formula: V = π/6lwd, where l is length, w is width, and d is depth. Imaging studies
started 21 days after tumor inoculation.

Edmonds S., Volpe A., Shmeeda H., Parente-Pereira A.C., Radia R., Baguña-Torres J., Szanda I., Severin G.W., Livieratos L., Blower P.J., Maher J., Fruhwirth G.O., Gabizon A, & T. M. de Rosales R. (2016). Exploiting the Metal-Chelating Properties of the Drug Cargo for In Vivo Positron Emission Tomography Imaging of Liposomal Nanomedicines. ACS Nano, 10(11), 10294-10307.

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Limiting dilution assay for tumor initiating cells

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For the limiting dilution assay, 8-weeks old female NOD scid gamma mice (NSG, Charles River) were subcutaneously injected with 1.5×10⁶ SUM159 cells resuspended in 100 μL of PBS. Mice were fed with standard diet or underwent FMD cycles (4 consecutive days per week) and were daily treated with 2DG (500mg/kg, via intraperitoneal injection) or vehicle. Body weights were recorded daily, and tumor volumes were measured twice a week by a digital caliper. After 5 weeks, donor mice were sacrificed and tumor masses were excised and digested, as previously described. Tumor cells derived from donor mice were re-injected at different dilution (100.000, 10.000, 1000 cells) in recipient 8-weeks old female NOD scid gamma mice. Recipient mice were always fed with standard diet and any drug wasn’t administered; curves were performed considering the percentage of tumor palpability. Tumor initiating cell frequency was calculated with ELDA software.

Salvadori G., Zanardi F., Iannelli F., Lobefaro R., Vernieri C, & Longo V.D. (2021). Fasting-mimicking diet blocks triple-negative breast cancer and cancer stem cell escape. Cell metabolism, 33(11), 2247-2259.e6.

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Establishing Patient-Derived Xenograft Models

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Hepatic metastatic samples from core needle biopsies were freshly collected into RPMI medium and added with pen/strep 1:100 v/v. Tumour samples, free from fat and necrotic tissue, were cut into small pieces of 3 x 3 x 3 mm and embedded in Matrigel (Corning Matrigel Basement Membrane Matrix, #354234). Five- to 6-week-old female Nod Scid Gamma mice (NSG) [strain NOD.Cg-Prkdc^scid Il2rg^tm1Wji/SzJ] provided by Charles River were anaesthetised using isoflurane gas anaesthesia and administered with buprenorphine dosed at 0.2 mg/kg. Subsequently, each piece of the tumour sample was implanted subcutaneously, by using an 18-gauge trocar, in one flank of the lower back of the mice. Due to the scarcity of the tumour samples proceeding from liver biopsies, in this first phase (F1, engraftment phase), only 2 or 3 NSG were used to be implanted.

Huebner A., Black J.R., Sarno F., Pazo R., Juez I., Medina L., Garcia-Carbonero R., Guillén C., Feliú J., Alonso C., Arenillas C., Moreno-Cárdenas A.B., Verdaguer H., Macarulla T., Hidalgo M., McGranahan N, & Toledo R.A. (2023). ACT-Discover: identifying karyotype heterogeneity in pancreatic cancer evolution using ctDNA. Genome Medicine, 15, 27.

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