Phenotyping of cell surface markers was performed by flow cytometry. The cells were stained with CD34-PE and CD45-FITC (all from BD Biosciences, USA), CD90-FITC (Dako, USA), CD73 PE (Abcam, USA) and isotype controls IgG1-FITC (Dako, USA), IgG1-PE (BD Biosciences, USA), and IgG2A-APC (BD Biosciences, USA). Flow cytometry data were acquired using a Guava EasyCyte 8HT flow cytometer and analysed using ExpressPro software (Merck Millipore, USA) comparing unlabelled, marker-labelled and isotype control populations in FL-1, FL-2 and FL-4 channels.
Cd90 fitc
Manufactured by Agilent Technologies
Sourced in United States
CD90-FITC is a fluorescently labeled antibody that binds to the CD90 cell surface antigen. It is used for the identification and isolation of cells expressing CD90 in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Guava easycyte 8ht, by Merck Group (1 mentions)
Cd45 fitc, by BD (1 mentions)
Cd34 pe, by BD (1 mentions)
Igg1 pe, by BD (1 mentions)
Cd73 pe, by Abcam (1 mentions)
Igg1 fitc, by Agilent Technologies (1 mentions)
Expresspro software, by Merck Group (1 mentions)
Igg2a apc, by BD (1 mentions)
Cd73 pe, by BioLegend (1 mentions)
Cd14 fitc, by Agilent Technologies (1 mentions)
Facscanto device, by BD (1 mentions)
Cd45 fitc, by Agilent Technologies (1 mentions)
2 protocols using cd90 fitc
1
Phenotyping Cell Surface Markers
2
Immunophenotyping of Mesenchymal Stem Cells
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The following surface markers were selected for the FACS analysis of the MSCs: CD14-FITC (Dako; dil. 1 : 50), CD45-FITC (Dako; dil. 1 : 50), CD73-PE (Biolegend, clone AD2; dil. 1 : 50), and CD-90-FITC (Dako; dil. 1 : 50). After 72 h of culturing, the MSCs were trypsinized and resuspended in the standard medium, whereupon 30 000 cells/sample were analysed. The cells were incubated with the appropriate antibody for 30 min at 4°C, then washed and resuspended in 300 μl of PBS. The FACS analysis was performed on a BD FACS CANTO device.
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