BAX samples were electrophoretically separated on 4–12% NuPage (Invitrogen) gels, transferred to mobilon-FL PVDF membranes (Millipore) and subjected to immunoblotting. For visualization of proteins with Odyssey Infrared Imaging System (LI-COR Biosciences) membranes were blocked in PBS containing 2.5% milk powder. Primary BAX yth-2d2 antibody (R&D systems) was incubated overnight at 4°C in a 1:1,000 dilution. After washing, membranes were incubated with an IRdye800-conjugated goat anti-mouse IgG secondary antibody (LI-COR Biosciences) in a 1:5,000 dilution. Protein was detected with Odyssey Infrared Imaging System. Densitometry of protein bands were acquired using a LI-COR Odyssey® scanner. Quantification and analysis was performed using the Western Analysis tool from the Image Studio 3.1 software.
Primary bax yth 2d2 antibody
Manufactured by R&D Systems
The Primary BAX yth-2d2 antibody is a laboratory reagent used in research applications. It is a specific antibody that recognizes the BAX protein, which is involved in the regulation of apoptosis, or programmed cell death. The antibody can be used to detect and study the expression and localization of the BAX protein in various cell and tissue samples.
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2 protocols using primary bax yth 2d2 antibody
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Quantitative Western Blot Analysis
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Quantitative Western Blot Analysis
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BAX samples were electrophoretically separated on 4–12% NuPage (Invitrogen) gels, transferred to mobilon-FL PVDF membranes (Millipore) and subjected to immunoblotting. For visualization of proteins with Odyssey Infrared Imaging System (LI-COR Biosciences) membranes were blocked in PBS containing 2.5% milk powder. Primary BAX yth-2d2 antibody (R&D systems) was incubated overnight at 4°C in a 1:1,000 dilution. After washing, membranes were incubated with an IRdye800-conjugated goat anti-mouse IgG secondary antibody (LI-COR Biosciences) in a 1:5,000 dilution. Protein was detected with Odyssey Infrared Imaging System. Densitometry of protein bands were acquired using a LI-COR Odyssey® scanner. Quantification and analysis was performed using the Western Analysis tool from the Image Studio 3.1 software.
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