Clarus 600 t gc ms

The total lipids were transesterified into fatty acid methyl esters (FAMEs) using the acid-catalyzed method [20 (link)]. The separation, identification and quantitation of the FAMEs were carried out by gas chromatography–mass spectrometry (GC–MS), using a PerkinElmer Clarus 600 T GC–MS (PerkinElmer, Inc., Shelton, CT, USA) [21 (link)]. The samples (1 μL) were injected into a Supelcowax 10 (60 m × 0.25 mm i.d., 0.25 μm film thickness; Supelco Inc., Bellefonte, PA, USA) capillary column in the split injection mode (split ratio 1:24). Helium was used as carrier gas with a flow rate of 0.8 mL min⁻¹. The GC program was as follows: initial temperature, 140 °C; increase by 7 °C min⁻¹ to 220 °C; and hold for 23 min. The injector temperature was set at 210 °C. The mass spectra were recorded in the positive-ion mode at 70 eV and a trap current of 100 μA with a source temperature of 150 °C. The mass scans were performed within the range of m/z 22–395 (0.14 scan s⁻¹ with an intermediate time of 0.02 s between the scans). FAMEs were identified by comparison of their retention times with those of the authentic standards (37 component FAME Mix, Supelco no. 47885-U) and the resulting mass spectra to those in the database (NIST MS Search 2.0). The amount of each fatty acid was calculated as peak area percentage of total fatty acids.

Hemp oil extraction was used in this experiment to determine the fatty acids in the seeds. The chemicals used for the preparation of fatty acid methyl esters (FAMEs) were purchased from Sigma-Aldrich (Steinheim, Germany), while the FAMEs standard (37 component FAME Mix, SUPELCO) was from Supelco (Bellefonte, PA, USA). The results obtained were checked against the dry matter.
Fatty acid methyl esters (FAMEs) of the total lipids (0.2 g oil) were produced via acid-catalyzed transesterification using 1% sulphuric acid in methanol [30 (link),31 (link)].
The methylated fatty acids were determined with a gas chromatograph (GC) coupled to a mass spectrometer (MS) (PerkinElmer Clarus 600 T GC-MS; Shelton, CT, USA) [32 (link)]. A 0.5 μL sample was injected into a 60 m × 0.25 mm i.d., 0.25 μm film thickness SUPELCOWAX 10 capillary column (Supelco Inc.). The operation conditions were as follows: injector temperature 210 °C; helium carrier gas flow rate 0.8 mL/min; split ratio 1:24; oven temperature 140 °C (hold 2 min) to 220 °C at 7 °C/min (hold 19 min); electron impact ionization voltage 70 eV; trap current 100 μA; ion source temperature 150 °C; mass range 22–395 m/z (0.14 scans/s with an intermediate time of 0.02 s between the scans).
The amount of each fatty acid was expressed as area percentages calculated from the total area of identified FAMEs.

An aliquot (10–15 mg) of the tomato seed oils was transesterified into FAMEs, using an acid-catalyzed procedure, as described previously [34 (link)] and analyzed by GC-MS. A PerkinElmer Clarus 600 T GC–MS (PerkinElmer, Inc., Shelton, CT, USA) system was used, equipped with a Supelcowax 10 capillary column (60 m × 0.25 mm i.d., 0.25 μm film thickness; Supelco Inc., Bellefonte, PA, USA). The column temperature was programmed from 140 to 220 °C at a rate of 7 °C/min and held for 23 min. The carrier gas (helium) was adjusted to a constant flow rate of 0.8 mL/min and the mass spectra were recorded in EI (positive ion electron impact) mode. The mass scans were performed from 22 to 395 m/z. Identification of the fatty acids was made by comparing peak areas with known standards and compounds listed in an MS database using NIST MS Search 2.0 software.
The quantitative analysis of FAMEs was performed using the total ion current chromatograms and the prevalence (%) of each fatty acid was computed as follows:
where IFA is individual fatty acid area and TAFA is total area of fatty acids.