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2 20 azino bis 3 ethylbenothiazoline 6 sulphonic acid diammonium salt

Manufactured by Merck Group
Sourced in United States

2,20-azino-bis(3-ethylbenothiazoline-6-sulphonic acid) diammonium salt is a chemical compound commonly used as a substrate in colorimetric enzyme-linked immunosorbent assays (ELISA) and other biochemical applications. It is a stable and water-soluble compound that undergoes a color change reaction when oxidized, allowing for the quantification of various analytes in a sample.

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2 protocols using 2 20 azino bis 3 ethylbenothiazoline 6 sulphonic acid diammonium salt

1

Comprehensive Characterization of Tea Polyphenols

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Tannase (200 U/g) was purchased from Yuanye Biological Technology Co. Ltd., Shanghai, China. The standard chemicals, including gallic acid, gallocatechin (GC), EGC, catechin, chlorogenic acid, caffeine, EGCG, EC, gallocatechin gallate (GCG), ECG, catechin gallate, ellagic acid, myricetin, quercitrin, and astragalin were purchased from Derick Biotechnology Co. Ltd., Chengdu, China. Formic acid and methanol were of chromatographic grade and obtained from Kermel Chemical Factory, Tianjin, China. The 2,20-azino-bis(3-ethylbenothiazoline-6-sulphonic acid) diammonium salt (ABTS), 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich, Saint Louis, MO, USA. All the other chemicals or reagents were of analytical grade. The deionized water was used for all experiments.
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2

Evaluation of Antioxidant Biomarkers

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Ethanol, acetic acid, and sodium chloride were obtained from Damao Chemical Reagent Factory (Tianjin, China). Polyformaldehyde was purchased from Wuhan Good Biotechnology Co., Ltd. (Wuhan, China). 2,4,6-Tripyridyl-S-triazine (TPTZ), Folin and Ciocalteu’s phenol, Trolox, and 2,20-azino-bis (3-ethylbenothiazoline-6-sulphonic acid) diammonium salt (ABTS) were obtained from Sigma-Aldrich (Saint Louis, MO, United States). All chemicals were of analytical grade, and the water used in this study was double distilled water. Detection kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), including triglyceride (TG), catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), malondialdehyde (MDA), and total protein assay kits.
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