Multi species leptin ria kit

Manufactured by Linco Research

Sourced in United States

The Multi-Species Leptin RIA kit is an in-vitro diagnostic assay designed for the quantitative measurement of leptin in various animal species. The kit utilizes a radioimmunoassay (RIA) technique to determine the concentration of leptin in biological samples, such as serum or plasma. The core function of this product is to provide a reliable and standardized method for researchers to assess leptin levels across multiple animal species.

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Lab products found in correlation

Diagnostic kit, by Roche (2 mentions) Porcine insulin ria kit, by Linco Research (2 mentions) Sterile vacutainer, by BD (1 mentions) Free fatty acid quantification kit, by Abcam (1 mentions)

Close spelling variants of this product

Leptin RIA RIA kit Leptin

6 protocols using multi species leptin ria kit

Bovine Metabolic Biomarker Analysis

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At the end of growing, fattening and finishing periods, blood samples (20 mL) were taken from the jugular veins of steers using syringe and then transferring the blood into heparin coated sterile vacutainer (Becton Dickinson, NJ, USA) wrapped with foil. Next, plasma was harvested by centrifugation (3,000 rpm, 20 min). All harvested plasma was rapidly frozen and stored at −70°C until further assay.
Blood biochemical components including aspartic acid transaminase (AST), alanine transaminase, blood urea nitrogen (BUN), glucose, total protein, albumin, cholesterol, and triglyceride were analyzed with the Automatic Biochemical Analyzer (HI System, Technicon, Tarrytown, NY, USA).
Plasma insulin and leptin were assayed by enzyme-immunoassay using Multi-species Insulin RIA kit and Multi-species Leptin RIA kit (Linco Research, Inc, St. Charles, MO, USA), respectively. I125 radioactivity was monitored using γ-counter (COBRATM II, Packard Bioscience, Downers Grove, IL, USA).

Chung C.S., Cho W.K., Jang I.S., Lee S.S, & Moon Y.H. (2017). Effects of feeding system on growth performance, plasma biochemical components and hormones, and carcass characteristics in Hanwoo steers. Asian-Australasian Journal of Animal Sciences, 30(8), 1117-1123.

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Comprehensive Lipid Profile and Insulin Resistance Evaluation

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Total cholesterol, high-density lipoprotein cholesterol (HDL-cholesterol), low-density lipoprotein cholesterol (LDL-cholesterol), triacylglycerols (TAG), phospholipids, total protein, urea, glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT), gamma-glutamyltransferase (GGT) and alkaline phosphatase (ALP) were analysed through diagnostic kits (Roche Diagnostics, Mannheim, Germany), using a Modular Hitachi Analytical System (Roche Diagnostics). Very low-density lipoprotein cholesterol (VLDL-cholesterol) and total lipids were calculated by Friedewald et al. [54 (link)] and Covaci et al. [55 ] formulas, respectively. Insulin and leptin concentrations were determined through the Porcine Insulin RIA kit (PI-12 K, Linco Research, Millipore, MA, USA) and the Multi-Species Leptin RIA kit (XL-85 K, Linco Research), respectively. To calculate the degree of insulin resistance, it was used the homeostasis model assessment using the insulin resistance index (HOMA-IR): fasting plasma glucose (mmol/L) times fasting plasma insulin (mU/L) divided by 22.5 [56 (link)]. Low HOMA-IR values indicate high insulin sensitivity, while high HOMA-IR values indicate high insulin resistance [56 (link)].

Madeira M.S., Rolo E.S., Pires V.M., Alfaia C.M., Coelho D.F., Lopes P.A., Martins S.I., Pinto R.M, & Prates J.A. (2017). Arginine supplementation modulates pig plasma lipids, but not hepatic fatty acids, depending on dietary protein level with or without leucine. BMC Veterinary Research, 13, 145.

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Hormonal Assay Protocols in Research

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Serum insulin levels were measured using a radioimmunoassay (rInsulin [I-125] RIA kit; Institute of Isotopes Co., Ltd., Tokyo, Japan); the sensitivity of the assay was 0.06 ng/mL. Serum leptin levels were measured using another radioimmunoassay kit (multi-species leptin RIA kit; Linco Research, Inc., St. Charles, MO, USA); the sensitivity of the assay was 1.0 ng/mL, and the inter-and intra-assay coefficients of variation (CVs) were 3.2% and 7.8%, respectively. Serum estradiol (E2) and testosterone levels were measured by a commercial laboratory (SRL, Tokyo, Japan) using an electrochemiluminescence immunoassay (Roche Diagnostics GmbH). Serum luteinizing hormone (LH) levels were measured using a radioimmunoassay (rLH [I-125] RIA kit; Institute of Isotopes Co., Ltd.); the sensitivity of the assay was 0.8 ng/mL, and the inter-and intra-assay CV were 7.7% and 6.5%, respectively. Serum follicle-stimulating hormone (FSH) levels were measured using a radioimmunoassay (rFSH [I-125] RIA kit; Institute of Isotopes Co., Ltd.); the sensitivity of the assay was 0.09 ng/mL and the inter-and intra-assay CV were 10.3 and 8.4%, respectively.

Iwasa T., Matsuzaki T., Yano K., Mayila Y., Yanagihara R., Yamamoto Y., Kuwahara A, & Irahara M. (2018). Prenatal undernutrition affects the phenotypes of PCOS model rats. The Journal of endocrinology, 239(2).

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Leptin Quantification in Cell Culture

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Concentrations of leptin were determined in incubation medium by using a multi-species leptin RIA kit (LINCO Research, St. Charles, USA) according to the instructions of the manufacturer.
RIA was validated for use in samples of culture medium. The cross-reactivity of leptin antiserum to human leptin was 100%, while to porcine, rat, mouse, and canine leptins was 67%, 61%, 73%, and 3%, respectively. Its cross reactivity to human insulin, human proinsulin, human C-peptide, rat insulin, glucagon, and IGF-I was less than 0.001%. Leptin assay sensitivity was 0.801 ng/ml. Intra and interassay coefficients of variation did not exceed 3.6% and 8.7 %, respectively. RIA was validated for use in samples of culture medium by dilution tests.

Štochmal'ová A., Harrath A.H., Alwasel S, & Sirotkin A.V. (2019). Direct inhibitory effect of flaxseed on porcine ovarian granulosa cell functions. Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme, 44(5).

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Comprehensive Lipid and Metabolic Profiling

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Total cholesterol, HDL-cholesterol, LDL-cholesterol, TAG, phospholipids, total proteins, urea, N and glucose concentrations, aspartate aminotransferase, alanine aminotransferase (ALT), γ-glutamyltransferase (GGT) and alkaline phosphatase (ALP) were analysed using diagnostic kits (Roche Diagnostics) and a Modular Hitachi Analytical System (Roche Diagnostics). VLDL-cholesterol and total lipids were calculated as described by Friedewald et al. (23) and Covaci et al. (24) , respectively. Free fatty acids (FFA) were quantified using the Free Fatty Acid Quantification Kit (BioVision Inc.). Insulin and leptin concentrations were determined through the Porcine Insulin RIA kit (PI-12K; Linco Research) and the Multi-Species Leptin RIA kit (XL-85K; Linco Research), respectively. The degree of insulin resistance was calculated by the homoeostasis model assessment using the insulin resistance index (HOMA-IR): fasting serum glucose (mmol/l) times fasting serum insulin (mU/l) divided by 22•5 (25)

Madeira M.S., Pires V.M., Alfaia C.M., Lopes P.A., Martins S.V., Pinto R.M, & Prates J.A. (2016). Restriction of dietary protein does not promote hepatic lipogenesis in lean or fatty pigs. The British journal of nutrition, 115(8).

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Plasma Metabolite Quantification Methods

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Plasma concentrations of glucose (glucose oxidase/peroxidase method) were determined with an automatic analyzer (GernonStar; RAL/TRANSASIA, Dabhel, India). The reagents for the glucose analyses were provided by the manufacturer of the analyzer (RAL Técnica para el Laboratorio, S.A., Barcelona, Spain). The intra-and interassay CV were 1.5 and 0.9%, respectively. The sensitivity was 0.056 mmol/L. Circulating IGF-I concentrations were quantified with a solid-phase EIA assay (Immulite; Siemens Medical Solutions Diagnostics Limited, Llanberis, Gwynedd, UK). The mean intra-and interassay CV were 3.1 and 12.0%, respectively. The sensitivity of the technique was 20 ng/mL. Plasma leptin concentrations were determined using RIA with a multispecies commercial kit (Multispecies Leptin Ria kit; Linco Research, Inc., St. Charles, MO). The mean intra-and interassay CV were 3.5 and 6.9%, respectively. The sensitivity was 1.30 ng/mL.

Dervishi E., Blanco M., Rodríguez-Sánchez J.A., Sanz A., Calvo J.H, & Casasús I. (2017). Milk yield and genomewide expression profiling in the mammary gland of beef primiparous cows in response to the dietary management during the pre- and postweaning periods. Journal of animal science, 95(10).

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