Kaempferol

Manufactured by Bio-Techne

Sourced in United Kingdom

Kaempferol is a natural compound found in various plant species. It is a flavonoid with antioxidant properties. Kaempferol is commonly used as a reference standard in analytical testing and research applications.

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Lab products found in correlation

3 protocols using kaempferol

Cell culture and treatments for insulinoma study

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The mouse
insulinoma cell
lines βTC6 (ATCC) and MIN6 were maintained in Dulbecco’s
modified Eagle’s medium (DMEM) supplemented with 15% fetal
bovine serum (FBS), 1× GlutaMAX, 1× nonessential amino acids,
and 1 mM sodium pyruvate. INS-1 rat insulinoma cells were maintained
in RPMI medium supplemented with 10% FBS, 1× GlutaMAX, 1×
nonessential amino acids, and 1 mM sodium pyruvate. Human islets were
obtained from the Integrated Islet Distribution Program (Duarte, CA)
in accordance with Oklahoma Medical Research Foundation internal review
board (IRB) and ethical guidelines for the use of human tissue. Standard
viability was 80–90% and purity was >80%. Islets were maintained
in CMRL medium supplemented with 10% FBS. All cells were grown at
37 °C in a humidified 5% CO₂ atmosphere. Tunicamycin
(Tm), brefeldin A (BFA), and thapsigargin (Tg) were from Sigma. CellTiter-Glo
and Caspase-Glo 3/7 reagents were from Promega. Salubrinal, guanabenz
acetate, STF083013, and kaempferol were from Tocris, quercetin and
apigenin were from Cayman, and PP1 Analog II was from Calbiochem.

Tran K., Li Y., Duan H., Arora D., Lim H.Y, & Wang W. (2014). Identification of Small Molecules That Protect Pancreatic β Cells against Endoplasmic Reticulum Stress-Induced Cell Death. ACS Chemical Biology, 9(12), 2796-2806.

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Screening of Bioactive Natural Products

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Luteolin was purchased from Santa Cruz Biotechnology (Santa Cruz, CA) and kaempferol was purchased from Tocris Bioscience (Ellisville, MO). Chrysin, Apigenin, Galangin, and other chemicals, unless otherwise indicated, were purchased from Sigma (St. Louis, MO). The collection of bioactive natural products used for screening was prepared from the Spectrum collection of MicroSource Discovery Systems, Inc. (Gaylordsville, CT). HCO3^--buffered solution containing (in mM): 120 NaCl, 5 KCl, 1 MgCl₂, 1 CaCl₂, 10 D-glucose, 2.5 HEPES, and 25 NaHCO₃ (pH 7.4). The half-Cl^- solution was prepared by replacing 65 mM NaCl with equimolar Na-gluconate in the HCO₃^--buffered solution.

Seo Y., Ryu K., Park J., Jeon D.K., Jo S., Lee H.K, & Namkung W. (2017). Inhibition of ANO1 by luteolin and its cytotoxicity in human prostate cancer PC-3 cells. PLoS ONE, 12(3), e0174935.

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Phytochemical Compounds from Reputable Sources

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The following compounds, with purity between 90–98% according to the manufacturer, were obtained from Sigma (St. Louis, MO): hydroxytyrosol, baicalein, cis‐2‐decenoic acid, morin, oenin, vitamin D3 (1,25‐dihydroxycholecalciferol) and vitamin C (ascorbic acid). The following compounds, with purity between 97–99% according to the manufacturer, were purchased from Tocris Bioscience (Bristol, UK): rosmarinic acid, kaempferol, piceatannol, rottlerin, luteolin and fisetin. Other reagents used in this study were organic kelp with standardized iodine content (i.e. 150 μg ml⁻¹ as 100% Daily Value) purchased from World Organic Ltd. (Auckland, New Zealand), and monolaurin (Lauricidin^®) purchased from Med‐Chem Laboratories, Inc., (Goodyear, AZ) as a pure sn‐1 monolaurin (glycerol monolaurate) derived from coconut oil.

Goc A., Niedzwiecki A, & Rath M. (2015). In vitro evaluation of antibacterial activity of phytochemicals and micronutrients against Borrelia burgdorferi and Borrelia garinii. Journal of Applied Microbiology, 119(6), 1561-1572.

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