ROT was purchased from Sigma-Aldrich (R8875, St. Louis, MO, USA), Madopar® was purchased from Roche Pharmaceutical Co., Ltd. (Shanghai, China), and sunflower oil was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (S24927, Shanghai, China). Rabbit anti-tyrosine hydroxylase (TH) primary antibody, mouse anti-alpha-synuclein (α-syn) antibody, rabbit anti-catechol-O-methyltransferase (COMT) antibody, rabbit anti-monoamine oxidase B (MAO-B) antibody, rabbit anti-dopa decarboxylase (DDC) antibody, and rabbit anti-heme oxygenase-1 (HO-1) antibody were all purchased from Abcam (ab137869, ab280377, ab126618, ab259928, ab131282, and ab68477, respectively; Cambridge, UK). Mouse anti-β-actin antibody was purchased from Huaxing Bio Specialist in Biological Medicine (HX1827; Beijing, China), rabbit anti-nuclear factor erythroid 2-related factor 2 (Nrf2) antibody was purchased from Immunoway (YT3189; Plano, TX, USA), and secondary antibodies linked to fluorescein (FITC)-conjugated AffiniPure Donkey Anti-Rabbit IgG (H + L) were purchased from Proteintech (SA00003-8, Wuhan, China).
Nrf2 antibody
Manufactured by Immunoway
Sourced in United States
The Nrf2 antibody is a laboratory tool used for the detection and analysis of the Nrf2 protein. Nrf2 is a transcription factor that plays a key role in the cellular response to oxidative stress. The antibody can be used in various techniques, such as Western blotting, immunohistochemistry, and immunoprecipitation, to study the expression and localization of Nrf2 in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Ab137869, by Abcam (1 mentions)
Ab280377, by Abcam (1 mentions)
Ab126618, by Abcam (1 mentions)
Ab68477, by Abcam (1 mentions)
Fitc conjugated affinipure donkey anti rabbit igg h l, by Proteintech (1 mentions)
Th primary antibody, by Abcam (1 mentions)
B27 supplement, by Thermo Fisher Scientific (1 mentions)
General reagents, by Merck Group (1 mentions)
Leptomycin b, by Beyotime (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
Curcumin, by Merck Group (1 mentions)
Lactate dehydrogenase ldh kit, by Beyotime (1 mentions)
2 protocols using nrf2 antibody
1
Immunohistochemical Analysis of Parkinsonian Markers
2
Neuroprotective Effects of GSK-3β Inhibition
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Adult male Sprague-Dawley rats (250-300 g) were used for the in vivo study. Newborn Sprague-Dawley rats (less than 24 h old) were used in the in vitro study. All experiments were approved by the Institutional Animal Care and Use Committee of the Chongqing Medical University.
GSK-3β lentivirus was purchased from Neuronbiotech (Shanghai, China). Leptomycin B and a lactate dehydrogenase (LDH) kit were purchased from Beyotime Biotechnology (Shanghai, China). Curcumin was used as a positive control and obtained from Sigma-Aldrich (St Louis. MO. USA). Nrf2 antibody was obtained from ImmunoWay (Newark, DE, USA). Neurobasal Medium, supplement B27, and glucose-free Dulbecco's Modified Eagle's Medium (DMEM) were purchased form Gibco BRL (Grand Island, NY, USA). MTS and 2,3,5-triphenyl tetrazolium chloride (TTC) were obtained from Sigma-Aldrich. General reagents were purchased from Sigma-Aldrich and other commercial suppliers.
GSK-3β lentivirus was purchased from Neuronbiotech (Shanghai, China). Leptomycin B and a lactate dehydrogenase (LDH) kit were purchased from Beyotime Biotechnology (Shanghai, China). Curcumin was used as a positive control and obtained from Sigma-Aldrich (St Louis. MO. USA). Nrf2 antibody was obtained from ImmunoWay (Newark, DE, USA). Neurobasal Medium, supplement B27, and glucose-free Dulbecco's Modified Eagle's Medium (DMEM) were purchased form Gibco BRL (Grand Island, NY, USA). MTS and 2,3,5-triphenyl tetrazolium chloride (TTC) were obtained from Sigma-Aldrich. General reagents were purchased from Sigma-Aldrich and other commercial suppliers.
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