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Hli373

Manufactured by Merck Group
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Sourced in Canada

The HLI373 is a laboratory equipment product manufactured by Merck Group. It is designed for general purpose use in research and testing environments. The core function of the HLI373 is to perform various analytical and measurement tasks, though the specific details of its intended use are not included in this factual description.

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Lab products found in correlation

Mg132, by Bio-Techne (2 mentions) S dhpg, by Bio-Techne (2 mentions) Anti ubiquitin monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)

3 protocols using hli373

1

In Vitro MDM2 Ubiquitination Assay

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Experiments were performed as previously described [51 (link)]. The ubiquitination reactions contained 0.1 ug of E1, 0.2 ug of UbE2D2, 5 ug of ubiquitin, and 0.5 ug of E3 (His-tagged MDM2) were performed in a volume of 20 uL for 90 min at 30 °C. Hinokiflavone or MDM2 inhibitor HLI373 (Sigma #373226, Canada) was added to the reaction at the indicated concentrations. Total ubiquitination was detected by immunoblotting using a monoclonal anti-ubiquitin antibody (Santa Cruz, sc-8017). MDM2 ubiquitination was visualized using an MDM2-specific antibody (Santa Cruz, sc-965).
Ilic V.K., Egorova O., Tsang E., Gatto M., Wen Y., Zhao Y, & Sheng Y. (2022). Hinokiflavone Inhibits MDM2 Activity by Targeting the MDM2-MDMX RING Domain. Biomolecules, 12(5), 643.
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2

Pharmacological Modulation of Neuronal mGluR

Cited 1 time
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S-DHPG (Tocris, Mineapolis, MN) was used at 50 μM to activate mGluR in neurons.
MG132 (Tocris, Mineapolis, MN), a preoteasome inhibitor was used at concentration of 10 uM.
HLI373 (Sigma, St. Louis, MO), a Hdm2 E3 Ligase Inhibitor was used at a concentration of 5μM (Kitagaki et al., 2008 (link)).
Ifrim M.F., Janusz-Kaminska A, & Bassell G.J. (2022). Development of single-molecule ubiquitination mediated fluorescence complementation to visualize protein ubiquitination dynamics in dendrites. Cell reports, 41(7), 111658.
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3

Pharmacological Modulation of Neuronal mGluR

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
S-DHPG (Tocris, Mineapolis, MN) was used at 50 μM to activate mGluR in neurons.
MG132 (Tocris, Mineapolis, MN), a preoteasome inhibitor was used at concentration of 10 uM.
HLI373 (Sigma, St. Louis, MO), a Hdm2 E3 Ligase Inhibitor was used at a concentration of 5μM (Kitagaki et al., 2008 (link)).
Ifrim M.F., Janusz-Kaminska A, & Bassell G.J. (2022). Development of single-molecule ubiquitination mediated fluorescence complementation to visualize protein ubiquitination dynamics in dendrites. Cell reports, 41(7), 111658.
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