Cellular lysates were loaded onto a 4-15% precast Criterion polyacrylamide gel (Biorad). The separated proteins were transferred onto PVDF membranes (Millipore), blocked for 1 hr at room temperature with PBS-SEA Block buffer (Thermo Scientific). The PVDF membranes were then incubated with primary antibodies against GFP(YFP) (clone B-2, Santa Cruz Biotechnology, GRB2 (clone 23, Santa Cruz Biotechnology), LCK (Cell Signaling), Actin (clone C4, Millipore), or GAPDH (Meridian Life Sciences). Secondary antibodies conjugated to IRDye 800CW or IRDye 680 were diluted in SEA Block and incubated with the PVDF membranes for 30 min at room temperature. The membranes were then washed with PBS-0.1%SDS solution and then visualized using the Licor Odyssey Infrared detector.
Gfp yfp clone b 2
Manufactured by Santa Cruz Biotechnology
The GFP(YFP) (clone B-2) is a lab equipment product designed for research purposes. It is a variant of the green fluorescent protein (GFP) with yellow-shifted emission. The core function of this product is to serve as a fluorescent marker for gene expression and protein localization studies in a variety of cell types.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Merck Group (2 mentions)
Criterion polyacrylamide gel, by Bio-Rad (2 mentions)
Pvdf membrane, by Santa Cruz Biotechnology (2 mentions)
Actin clone c4, by Merck Group (2 mentions)
Grb2 clone 23, by Santa Cruz Biotechnology (2 mentions)
Pbs sea block buffer, by Thermo Fisher Scientific (2 mentions)
2 protocols using gfp yfp clone b 2
1
Western Blotting Protein Detection
2
Western Blotting Protein Detection
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Cellular lysates were loaded onto a 4-15% precast Criterion polyacrylamide gel (Biorad). The separated proteins were transferred onto PVDF membranes (Millipore), blocked for 1 hr at room temperature with PBS-SEA Block buffer (Thermo Scientific). The PVDF membranes were then incubated with primary antibodies against GFP(YFP) (clone B-2, Santa Cruz Biotechnology, GRB2 (clone 23, Santa Cruz Biotechnology), LCK (Cell Signaling), Actin (clone C4, Millipore), or GAPDH (Meridian Life Sciences). Secondary antibodies conjugated to IRDye 800CW or IRDye 680 were diluted in SEA Block and incubated with the PVDF membranes for 30 min at room temperature. The membranes were then washed with PBS-0.1%SDS solution and then visualized using the Licor Odyssey Infrared detector.
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