Fvs psu ix2 ucb

Fluorescence microscopy and high-resolution confocal reflection microscopy were performed using an Olympus IX81, FV300 (Olympus, Melville, NY) confocal-scanning microscope equipped with a 60×/1.42 NA oil objective for viewing PLTs. An Olympus FVS-PSU/IX2-UCB camera and scanning unit and Olympus Fluoview FV 300 image acquisition software version 5.0 were used for recording. In addition, an EVOS FL Auto Cell imaging system with an integrated dual camera system, system-specific software and equipped with a 609/1.42 NA oil objective was used. Monochrome 16-bit images were further analyzed, and changes were quantified using Adobe Photoshop CS6, ImageJ (NIH), and CellProfiler (www.cellprofiler.org (last accessed on 1 October 2022)) [96 (link)]. Analysis of images presented in Figure 5B was performed with a customized CellProfiler analysis set. In brief, raw images were automatically analyzed to minimize observer bias. The following software parameters were introduced: threshold method—otsu (this approach calculates the threshold separating three classes of pixels by minimizing the variance within each class); threshold correction factor—2.5 (a value > 1 makes the threshold more stringent, since the otsu method will give a slightly biased threshold if a larger percentage of the image is covered with positive signal).