Glutathione peroxidase gpx

Absolute ethanol (EtOH) solution was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Polyclonal rabbit anti-mouse iNOS antibody (1 : 100; Cat: ab15323), monoclonal rabbit anti-human Bax antibody (1 : 300; Cat: ab32503), polyclonal rabbit anti-mouse IL-1β antibody (1 : 250; Cat: ab9722), and polyclonal rabbit anti-human CD3 antibody (1 : 100; Cat: ab5690) were purchased from Abcam Co., Cambridge, UK.
Kits for malondialdehyde (MDA), GSH, total superoxide dismutase (T.SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione S-transferase (GST) were obtained from Biodiagnostic Co. (Dokki, Giza, Egypt). Total RNA extraction and SYBR Green Master Mix kits were purchased from Qiagen Co., Germany. cDNA kit was obtained from Promega Co., Madison, WI, USA.

Streptozotocin (STZ, 98% purity) was obtained from Sigma-Aldrich Chemical Company (St. Louis, MO, USA), as were total cholesterol (TC) and triglyceride (TG). Insulin was obtained from Linco Research, USA. Nitric oxide (NO) was obtained from Bio Systems S.A. Glucose, thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were obtained from Biodiagnostic Co. (Cairo, Egypt). Synaptophysin and Ki67 antibodies were obtained from Abcam Co. (Cambridge, UK). Other reagents were purchased from MyBioSource Co. (San Diego, USA). Daiwa Pharmaceutical Co. Ltd. (Tokyo, Japan) kindly provided Biobran (99% purity).