Cells were incubated in the presence of DS-SILY20 with or without 10 ng/mL PDGF for 24 hr. Media was removed from the cultures and a Pro-Inflammatory I kit (Meso Scale Discovery) was used to analyze cytokine production from EC monolayers, hyperplastic co-cultures, and quiescent co-cultures according to manufacturer’s instructions. Briefly, plates were warmed to room temperature and incubated with 25 μL of samples and standards for 2 hr at room temperature with vigorous shaking. The detection antibody was then added to the plate and incubated for 2 hr at room temperature with vigorous shaking. After washing three times with PBS with 0.05% Tween-20, 2X read buffer was added to the plate and imaged using a Sector Imager 2400A (Meso Scale Discovery). The pro-inflammatory markers interferon-γ (IFN-γ), interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were examined in this study. Data were analyzed using the MSD Discovery Workbench Software and cytokine production from cultures was normalized to cell viability.
Pro inflammatory 1 kit
Manufactured by Mesoscale
The Pro-Inflammatory I kit is a lab equipment product designed to measure and analyze pro-inflammatory markers. The kit provides the necessary reagents and materials to perform relevant assays.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pro inflammatory 1 kit
1
Cytokine Production in Endothelial Cell Models
2
Cytokine Profile of SMCs Under PDGF/IFN-γ
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were incubated
in the presence
of 10 ng/mL PDGF or IFN-γ with or without DS-SILY20 for 24 h. Media was removed from the cultures and a Pro-Inflammatory
I kit (Meso Scale Discovery) was used to analyze cytokine production
of SMCs according to manufacturer’s instructions. Briefly,
plates were warmed to room temperature and incubated with 25 μL
of samples and standards for 2 h at room temperature with vigorous
shaking. The detection antibody was then added to the plate and incubated
for 2 h at room temperature with vigorous shaking. After washing three
times with PBS with 0.05% Tween-20, 2× read buffer was added
to the plate and imaged using a Sector Imager 2400A (Meso Scale Discovery).
The pro-inflammatory markers interleukin-1β(IL-1β), interleukin-6
(IL-6), and tumor necrosis factor-α(TNF-α) were examined
in this study. Data were analyzed using the MSD Discovery Workbench
Software.
in the presence
of 10 ng/mL PDGF or IFN-γ with or without DS-SILY20 for 24 h. Media was removed from the cultures and a Pro-Inflammatory
I kit (Meso Scale Discovery) was used to analyze cytokine production
of SMCs according to manufacturer’s instructions. Briefly,
plates were warmed to room temperature and incubated with 25 μL
of samples and standards for 2 h at room temperature with vigorous
shaking. The detection antibody was then added to the plate and incubated
for 2 h at room temperature with vigorous shaking. After washing three
times with PBS with 0.05% Tween-20, 2× read buffer was added
to the plate and imaged using a Sector Imager 2400A (Meso Scale Discovery).
The pro-inflammatory markers interleukin-1β(IL-1β), interleukin-6
(IL-6), and tumor necrosis factor-α(TNF-α) were examined
in this study. Data were analyzed using the MSD Discovery Workbench
Software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!