B16f0 mouse melanoma cells

Manufactured by American Type Culture Collection

Sourced in United States

B16F0 mouse melanoma cells are an established cell line derived from a spontaneous melanoma in a C57BL/6 mouse. They can be used for research purposes related to the study of melanoma.

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Lab products found in correlation

Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Human melanocyte growth supplement, by Thermo Fisher Scientific (1 mentions) Medium 254, by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Welgene (1 mentions) Hacat human keratinocytes, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions) Dulbecco modified eagle medium (dmem), by Welgene (1 mentions) Sk mel 28 human melanoma cells, by American Type Culture Collection (1 mentions) Z vad fmk, by Thermo Fisher Scientific (1 mentions) Cisplatin, by Merck Group (1 mentions)

2 protocols using b16f0 mouse melanoma cells

Culturing Melanoma, Melanocyte, and Keratinocyte Cells

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B16F0 mouse melanoma cells (ATCC, Manassas, VA, USA) were cultured in D MEM (Sigma-Aldrich) supplemented with 10% FBS (Hyclone) and penicillin-streptomycin solution (Hyclone). Human epidermal melanocytes (HEM, Thermo Fisher Scientific, Waltham, MA, USA) were cultured in Medium 254 (Thermo Fisher Scientific) containing Human Melanocyte Growth Supplement (HMGS, Thermo Fisher Scientific). MNT1 human melanoma cells (ATCC) were cultured in MEM (Welgene, Korea) containing 10% D MEM, 20% FBS, and penicillin-streptomycin solution. HaCaT human keratinocytes (Thermo Fisher Scientific) were cultured in D MEM supplemented with 10% FBS and penicillin-streptomycin. Human epidermal keratinocytes (HEK, Thermo Fisher Scientific) were cultured in Medium 254 (Thermo Fisher Scientific) containing Human Melanocyte Growth Supplement (HMGS, Thermo Fisher Scientific). These cells were incubated at 37 °C with 5% CO₂.

Yun C.Y., Choi N., Lee J.U., Lee E.J., Kim J.Y., Choi W.J., Oh S.H, & Sung J.H. (2021). Marliolide Derivative Induces Melanosome Degradation via Nrf2/p62-Mediated Autophagy. International Journal of Molecular Sciences, 22(8), 3995.

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Melanoma Cell Culture Protocols

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For in vitro studies, stock solutions of (−)-Nutlin-3 (30mM) and MLN8237 (20mM) were prepared in DMSO. The pan-caspase inhibitor Z-VAD-FMK was obtained from Molecular Probes (Eugene). Cisplatin was purchased from Sigma-Aldrich and a 10mM stock solution was prepared in DMSO. SK-Mel5, HS294T, SK-Mel28 human melanoma cells and B16F0 mouse melanoma cells were obtained from ATCC. Cells were cultured in DMEM/F12 media supplemented with 10% FBS, 100U/ml penicillin and 100ug/ml streptomycin.

Vilgelm A.E., Pawlikowski J.S., Liu Y., Hawkins O.E., Davis T.A., Smith J., Weller K.P., Horton L.W., McClain C.M., Ayers G.D., Turner D.C., Essaka D.C., Stewart C.F., Sosman J.A., Kelley M.C., Ecsedy J.A., Johnston J.N, & Richmond A. (2014). Mdm2 and Aurora A inhibitors synergize to block melanoma growth by driving apoptosis and immune clearance of tumor cells. Cancer research, 75(1), 181-193.

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