Evolutioncapt fx6

The qualitative analysis of 102 chemokines or cytokines was performed for CSF samples using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems) following the producer's protocol (Table 3). The membranes were incubated overnight with 300 μl of each sample and then washed and incubated with the detection cocktail, with streptavidin-conjugated horseradish peroxidase (R&D Systems) and finally with the chemiluminescence reagent. The spots were visualized by Fusion FX6 (Vilber Lourmat) with EvolutionCapt FX6. The registered signals were proportional to the amount of the bound analyte (Figure 2).

The analysis of 102 chemokines or cytokines was performed on CSF samples using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems) following manufacturers' protocol (Table 3). The kit consists of 4 membranes, which were incubated overnight with 300 μl of every sample and a binding buffer. The membranes were washed and incubated with the detection cocktail, with streptavidin-conjugated horseradish peroxidase (R&D Systems) for 30 min and finally incubation with the chemiluminescence reagent. The spots were visualized by Fusion FX6 (Vilber Lourmat) with EvolutionCapt FX6. The signal readings were proportional to the amount of the bound analyte. The results were analyzed by Zen Software (Zeiss) based on densitometric analysis (Figure 2). The graph bars present means from two spots captured with analyzed antibody according to the following formula: mean density of analyzed spots/mean density of control spots × 100%−mean density of background.