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Tet2 hpa 019032

Manufactured by Merck Group
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TET2 (HPA 019032) is a laboratory reagent produced by Merck Group. It is an antibody that specifically recognizes and binds to the TET2 protein. TET2 is an enzyme that plays a role in the epigenetic regulation of gene expression.

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Lab products found in correlation

Dako target retrieval solution, by Agilent Technologies (2 mentions) E cadherin hecd1, by Agilent Technologies (2 mentions) Tet1 sab 2501479, by Merck Group (2 mentions) Vimentin clone v9, by Agilent Technologies (2 mentions) Envision system, by Agilent Technologies (2 mentions)

2 protocols using tet2 hpa 019032

1

Immunohistochemical Analysis of Renal Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Specimens were formalin fixed and embedded in paraffin wax; 3-μm serial sections mounted on Snowcoat X-tra slides (Surgipath, Richmond, IL) were dewaxed in xylene and rehydrated using graded ethanol washes. For antigen retrieval, sections were immersed in preheated DAKO target retrieval solution (DAKO) and treated for 90 seconds in a pressure cooker. Sections analysed contained both tumour and adjacent normal renal parenchyma acting as an internal control; in addition, substitution of the primary antibody with antibody diluent was used as a negative control. Antigen/antibody complexes were detected using the Envision system (DAKO) according to the manufacturer's instructions. Sections were counterstained with hematoxylin for 30 seconds, dehydrated in graded ethanol washes, and mounted in DPX (Lamb, London, United Kingdom). Antibodies used were: E-cadherin (HECD1, CRUK) and vimentin (clone V9, Dako). TET1 (SAB 2501479) and TET2 (HPA 019032) antibodies were purchased by Sigma Aldrich.
Sciacovelli M., Gonçalves E., Isaac Johnson T., Roberto Zecchini V., da Costa A.S., Gaude E., Vercauteren Drubbel A., Julian Theobald S., Abbo S., Tran M., Rajeeve V., Cardaci S., Foster S., Yun H., Cutillas P., Warren A., Gnanapragasam V., Gottlieb E., Franze K., Huntly B., Richard Maher E., Henry Maxwell P., Saez-Rodriguez J, & Frezza C. (2016). Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition. Nature, 537(7621), 544-547.
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2

Immunohistochemical Analysis of Renal Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Specimens were formalin fixed and embedded in paraffin wax; 3-μm serial sections mounted on Snowcoat X-tra slides (Surgipath, Richmond, IL) were dewaxed in xylene and rehydrated using graded ethanol washes. For antigen retrieval, sections were immersed in preheated DAKO target retrieval solution (DAKO) and treated for 90 seconds in a pressure cooker. Sections analysed contained both tumour and adjacent normal renal parenchyma acting as an internal control; in addition, substitution of the primary antibody with antibody diluent was used as a negative control. Antigen/antibody complexes were detected using the Envision system (DAKO) according to the manufacturer's instructions. Sections were counterstained with hematoxylin for 30 seconds, dehydrated in graded ethanol washes, and mounted in DPX (Lamb, London, United Kingdom). Antibodies used were: E-cadherin (HECD1, CRUK) and vimentin (clone V9, Dako). TET1 (SAB 2501479) and TET2 (HPA 019032) antibodies were purchased by Sigma Aldrich.
Sciacovelli M., Gonçalves E., Isaac Johnson T., Roberto Zecchini V., da Costa A.S., Gaude E., Vercauteren Drubbel A., Julian Theobald S., Abbo S., Tran M., Rajeeve V., Cardaci S., Foster S., Yun H., Cutillas P., Warren A., Gnanapragasam V., Gottlieb E., Franze K., Huntly B., Richard Maher E., Henry Maxwell P., Saez-Rodriguez J, & Frezza C. (2016). Fumarate is an epigenetic modifier that elicits epithelial-to-mesenchymal transition. Nature, 537(7621), 544-547.
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