Hela, Siha, and C33a were all cultured in DMEM (HyClone, USA) culture medium, with 10% FBS (Gibco, USA), 1% penicillin streptomycin (Gibco, USA), 1% Mycoplasma Removal Agent (Yeasen Biotechnology, Shanghai), and were stored at 37 °C and a humidified atmosphere with 5% CO2 (Thermo Fisher, Germany). The TGF-beta activator SRI-011381 hydrochloride and the TGF-beta inhibitor LY2109761 were purchased from Selleck Chemicals.
Sri 011381 hydrochloride
Manufactured by Selleck Chemicals
SRI-011381 hydrochloride is a chemical compound used in laboratory research. It is a white crystalline powder that is soluble in water and various organic solvents. The compound has a molecular formula of C₁₅H₂₁NO₂·HCl and a molecular weight of 299.81 g/mol.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mycoplasma removal agent, by Yeasen (1 mentions)
Ly2109761, by Selleck Chemicals (1 mentions)
α sma antibody, by Boster Bio (1 mentions)
Collagen 1, by Abcam (1 mentions)
Collagen 3, by Abcam (1 mentions)
Nf200, by Abcam (1 mentions)
Smad2, by Santa Cruz Biotechnology (1 mentions)
P smad2, by Santa Cruz Biotechnology (1 mentions)
Tgf β1, by Santa Cruz Biotechnology (1 mentions)
Sb431542, by Selleck Chemicals (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using sri 011381 hydrochloride
1
TGF-beta Signaling Modulation in Cell Lines
2
TGF-β1 Signaling Pathway Modulation
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TGF-β1, TβRΙ, Smad2, p-Smad2 and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. Collagen I, Collagen III and NF-200 antibodies were purchased from Abcam. The α-SMA antibody was purchased from Boster Biological Technology. The TGF-β1/SMAD signaling agonist, SRI-011381 hydrochloride, and the inhibitor, SB-431542, were purchased from Selleck Chemicals unless otherwise specified.
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