Complete rpmi 1640 medium

Manufactured by PAN Biotech

Sourced in Germany

Complete RPMI 1640 medium is a cell culture medium formulation that provides the necessary nutrients and supplements for the growth and maintenance of a variety of cell types in vitro. It is a balanced salt solution that includes amino acids, vitamins, and other essential components required for cell proliferation and survival.

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Lab products found in correlation

Cell culture flask, by SPL Life Sciences (1 mentions) 25 cm2 cell culture flasks, by Thermo Fisher Scientific (1 mentions) Gentamycin solution, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Merck Group (1 mentions) Dmem high glucose medium, by PAN Biotech (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Fetal calf serum (fcs), by PAN Biotech (1 mentions) L glutamine, by PAN Biotech (1 mentions) Phytohemagglutinin (pha), by Thermo Fisher Scientific (1 mentions) Elispot plate, by BD (1 mentions) Ctl immunospot s5 uv analyzer, by Cellular Technology (1 mentions) Fetal bovine serum (fbs), by PAN Biotech (1 mentions)

Close spelling variants of this product

RPMI 1640 medium RPMI 1640 1640 medium RPMI medium

4 protocols using complete rpmi 1640 medium

Culturing Leishmania Parasites and Macrophages

Cited 1 time

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L. infantum (zymodeme GH8, strain MHOM/GR/2001/GH8) and L. major (zymodeme LV39, strain MRHO/SU/59/P) parasites, causative agents of visceral and cutaneous leishmaniasis, respectively, were used in this study. Promastigotes were grown at 26 °C in complete RPMI-1640 medium (PAN-Biotech, Aidenbach, Passau, Germany) in cell culture flasks (SPL Life Sciences, Naechon-Myeon, Pocheon-si, Korea), as previously reported [48 (link)].
The immortalized macrophage cell line J774A.1 (ATCC No: TIB-67) was cultured in 25 cm² cell culture flasks (ThermoFisher Scientific, Waltham, MA, USA), at 37 °C with a 5% CO₂ environment [48 (link)].

Gogou G., Koutsoni O.S., Stathopoulos P., Skaltsounis L.A., Halabalaki M, & Dotsika E. (2022). Direct In Vitro Comparison of the Anti-Leishmanial Activity of Different Olive Oil Total Polyphenolic Fractions and Assessment of Their Combined Effects with Miltefosine. Molecules, 27(19), 6176.

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Antiproliferative Evaluation of Bran Extracts

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The antiproliferative activity of bran extracts was tested on four cell lines—three of them are human cancer cells and one is the normal cell line. MCF-7 and T47D are human epithelial breast cancer cell lines, while MDA-MB-231 is human hormone-independent breast cancer cells. EMT6/P and fibroblast are the mouse epithelial breast cancer cell line and human skin fibroblast cells, respectively. To achieve a successful cell culture, many factors were followed such as using completed medium and incubating cells in 5% CO₂ at 37 °C. Based on the type of the cells, the type of culturing medium varied. For MCF-7 and T47D, complete RPMI 1640 medium (PAN-biotech, Aidenbach, Germany) was used, while complete MEM medium (PAN-biotech, Aidenbach, Germany) was used for culturing EMT6/P. High-glucose DMEM medium (PAN-biotech, Aidenbach, Germany) was utilized for MDA-MB-231 and fibroblast cell lines. In this context, a complete culture medium was prepared through adding the following supplements with the required percentage of each type of tissue culture medium. The supplements are: 1% L-glutamine (Sigma, St. Louis, MO, USA), 10% fetal bovine serum (Gibco, Brough, UK), 1% penicillin-streptomycin (Sigma, St. Louis, MO, USA), 0.1% non-essential amino acids (Sigma, St. Louis, MO, USA) and 0.1% gentamycin solution (Sigma, St. Louis, MO, USA).

Talib W.H., Mahmod A.I., Awajan D., Hamed R.A, & Al-Yasari I.H. (2022). Immunomodulatory, Anticancer, and Antimicrobial Effects of Rice Bran Grown in Iraq: An In Vitro and In Vivo Study. Pharmaceuticals, 15(12), 1502.

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Propagation of Trypanosoma cruzi Tulahuen

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In vivo passage of T. cruzi Tulahuen strain was achieved by i.p. inoculation of mice with 5 x 10⁵ bloodstream trypomastigotes resuspended in 200 μL of DPBS. Periodic passages took place every 15 days. For in vitro experiments, cell culture-derived T. cruzi trypomastigotes were obtained from the supernatant of infected 86Hg39 cells (BNITM) maintained in complete RPMI 1640 medium (PAN-BIOTECH) supplemented with 10 % of fetal calf serum (PAN-BIOTECH), 1 % L-Glutamine (PAN-BIOTECH), and 0.5 %, Gentamycin sulfate (PAA) at 37 °C and 95 % CO₂ after 3-4 days post-infection.

Arana Y., Gálvez R.I, & Jacobs T. (2022). Role of the PD-1/PD-L1 Pathway in Experimental Trypanosoma cruzi Infection and Potential Therapeutic Options. Frontiers in Immunology, 13, 866120.

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Cellular Immune Response Evaluation by ELISPOT

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Cellular immune responses were evaluated by ELISPOT assay, as described previously, with a few modifications [32 (link)]. Briefly, ELISPOT plates (BD Bioscience, USA) were coated with monoclonal anti-mouse interferon-gamma (IFN-γ) and interleukin-4 (IL-4) capture antibodies diluted in sterile PBS (5 μg/mL) and incubated at 4 °C overnight. Then, the coating antibody was discarded and blocked with complete RPMI 1640 medium (PAN Biotech, Bayern, Germany) with 10% fetal bovine serum (PAN Biotech, Bayern, Germany) and 1% Antibiotic-Antimycotic (Glibco, Burlington, MA, USA) for 2 h at RT. Freshly isolated splenocytes were seeded at a density of 1 × 10⁶ cells/well. Cells were stimulated with purified proteins, epitope-specific peptides (10 μg/well), or phytohemagglutinin as a positive control (Gibco, Burlington, MA, USA), or only medium maintain as a negative control. Next, plates were incubated for 48 h at 37 °C with 5% CO₂. Then, cells were discarded from the plates and sequentially treated with biotinylated anti-mouse IFN-γ and IL-4 antibodies, stereptavidin HRP, and substrate solution. The substrate reaction was terminated by washing with deionized water and dried in the dark at RT. Finally, Spots were enumerated by CTL-Immunospot S5 UV analyzer (Cellular technologies, Shaker Heights, OH, USA).

Chathuranga W.A., Hewawaduge C., Nethmini N.A., Kim T.H., Kim J.H., Ahn Y.H., Yoon I.J., Yoo S.S., Park J.H, & Lee J.S. (2022). Efficacy of a Novel Multiepitope Vaccine Candidate against Foot-and-Mouth Disease Virus Serotype O and A. Vaccines, 10(12), 2181.

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