Rabbit anti ps473akt

Manufactured by Cell Signaling Technology

Rabbit anti-pS473AKT is a primary antibody that recognizes the phosphorylated form of AKT at serine 473. It is commonly used in Western blotting and immunohistochemistry applications to detect and quantify the levels of activated AKT in cell and tissue samples.

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Lab products found in correlation

Rabbit anti akt, by Cell Signaling Technology (2 mentions) Rabbit anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions) Rabbit anti ng2, by Merck Group (1 mentions) Rat anti cd31 clone mec13.3, by BD (1 mentions) Mouse anti vinculin v9131, by Merck Group (1 mentions) Rabbit anti fak c 20, by Santa Cruz Biotechnology (1 mentions) Goat anti nrp1 c 19, by Santa Cruz Biotechnology (1 mentions) Rabbit anti gfap, by Agilent Technologies (1 mentions) Rat anti ve cadherin, by BD (1 mentions) Rabbit anti aif, by Cell Signaling Technology (1 mentions) Mouse anti total oxphos antibody cocktail, by Abcam (1 mentions) Rabbit anti micu1, by Merck Group (1 mentions) Mouse anti gfp, by Roche (1 mentions) Guinea pig anti p62, by Progen Biotechnik (1 mentions) Mouse anti mtco1, by Abcam (1 mentions) Mouse anti tom40, by Santa Cruz Biotechnology (1 mentions) Anti pras40, by Cell Signaling Technology (1 mentions) Rabbit anti tom20, by Proteintech (1 mentions) Mouse anti tubulin, by Merck Group (1 mentions) Rabbit anti gapdh, by Santa Cruz Biotechnology (1 mentions)

2 protocols using rabbit anti ps473akt

Recombinant Protein and Antibody Analysis

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Recombinant human VEGF₁₆₅ (Ala27-Arg191, no. 293-VE), human Sema3C (Gly21-Ser738, no. 5570-S3), and mouse Sema3C (Gln24-Ser741, no. 1728-S3) were from R&D Systems. Recombinant human IgG-Fc (009-000-008) was purchased from Jackson ImmunoResearch. Antibodies: rat anti-Sema3C (clone 238835) and goat anti-Nrp-1 (AF566) are from R&D Systems; rabbit anti-pY397FAK, rabbit anti-pT180/pY182-p38MAPK (Invitrogen); rabbit anti-FAK (C-20), and goat anti-Nrp-1 (C-19) are from Santa Cruz; mouse anti-p38MAPK (L53F8), rabbit anti-AKT, rabbit anti-pS473AKT, and rabbit anti-cleaved Caspase-3 (Asp175) are from Cell Signaling; pTyr118 paxillin (NEB), rat anti-CD34 (NCL-END; Novocastra); rat anti-CD31 (clone Mec13.3) and rat anti-VE-cadherin are from BD Biosciences; mouse anti-vinculin (V9131) and anti-actin smooth muscle-Cy3 are from Sigma; rabbit anti-GFAP (DAKO), rabbit anti-NG2 (Millipore), rabbit anti-collagen IV (Cosmo Bio).

Yang W.J., Hu J., Uemura A., Tetzlaff F., Augustin H.G, & Fischer A. (2015). Semaphorin-3C signals through Neuropilin-1 and PlexinD1 receptors to inhibit pathological angiogenesis. EMBO Molecular Medicine, 7(10), 1267-1284.

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Comprehensive Antibody Panel for OXPHOS

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The following antibodies were used in our work: rabbit anti-NDUFB8 (Proteintech, 1479-1-AP); mouse anti-MTCO1 (Abcam, ab14705); mouse anti-total OXPHOS antibody cocktail (Abcam, ab110413); mouse anti-GFP (Roche, 11814460001); rabbit anti-VPS39 (Proteintech, 16219-1-AP and Novus Biologicals, NBP1-76535); mouse anti-KDEL (Millipore, 10C3); mouse anti-TOM40 (Santa Cruz, sc-365467); rabbit anti-TOM20 (Proteintech, 11802-1-AP); rabbit anti-LC3B (Sigma, L7543); rabbit anti-MICU1 (Sigma, PA5-83371); guinea pig anti-p62 (Progen, GP62-C); rabbit anti-pS473-AKT (Cell Signaling, 4060S), rabbit anti-AKT (Cell Signaling, 4685); rabbit anti-pT246-PRAS40 (Cell Signaling, 13175); anti-PRAS40 (Cell Signaling, 2691); rabbit anti-GAPDH (Santa Cruz, sc-25778); mouse anti-actin (Sigma, A5316 and abcam, ab14128); mouse anti-tubulin (Sigma, T6074); rabbit anti-SGPL1 (Atlas Antibodies, HPA021125); rabbit anti-AIF (Cell Signaling, 5318).

Jackson J., Wischhof L., Scifo E., Pellizzer A., Wang Y., Piazzesi A., Gentile D., Siddig S., Stork M., Hopkins C.E., Händler K., Weis J., Roos A., Schultze J.L., Nicotera P., Ehninger D, & Bano D. (2022). SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells. Molecular Metabolism, 61, 101503.

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