A 2F pressure-volume (PV) conductance catheter (SPR-858, Millar Instruments, TX) was inserted into the left ventricle (LV) using the closed chested method (17 (link)). Briefly, the PV catheter was inserted through the exposed right carotid artery and slowly advanced into the LV. Pressure and volume signals were acquired continuously (MPVS300, Millar Instruments, Houston, TX, and PowerLab 8/30, AD Instruments, Colorado Springs, CO). LV volume was measured in conductance units (relative volume unit, RVU) and converted to absolute blood volume (μL) at the end of the experiment (17 (link)). Parallel volume (the contribution of the myocardium to the measured LV volume) was calibrated via IV injection of 35 μL hypertonic saline (15% NaCl w/v) (17 (link)).
Spr 858
Manufactured by Millar
The SPR-858 is a surface plasmon resonance (SPR) instrument designed for the study of molecular interactions. It measures changes in the refractive index at the surface of a sensor chip, allowing for the real-time analysis of biomolecular binding events.
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Lab products found in correlation
3 protocols using spr 858
1
Pressure-Volume Catheter Technique for Left Ventricular Measurements
2
Left ventricular PV loop measurement
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A 2F pressure–volume (PV) conductance catheter (SPR-858; Millar Instruments, Houston, Tex) was inserted into the left ventricle (LV) using the closed chested method and the signal acquired continuously (MPVS300; Millar Instruments, and PowerLab 8/30, ADInstruments, Colorado Springs, Colo) (28 (link)). For the closed chest method, the right carotid artery is isolated with blunt dissection, and the PV catheter is advanced through the aorta until a PV loop is acquired.
3
Cardiac Function Assessment in Rats
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Studies were performed in 200–250g male Sprague-Dawley rats (Harlan Laboratories, Indianapolis, IN). Animal handling and care followed NIH Guide for Care and Use of Laboratory Animals and all protocols were approved by the UC San Diego Institutional Animal Care and Use Committee. Animals were initially anesthetized with isoflurane (Draegerwerk AG, Luebeck, Germany) at 5% to induce deep anesthesia. Anesthetic was subsequently reduced to 2.5% for the remainder of the experiment, and animals were placed in the supine position on a heating pad to maintain core body temperature at . Catheters were placed in the left femoral artery and left jugular vein. Animals were then instrumented with a 2F pressure-volume (PV) conductance catheter (SPR858, Millar Instruments, TX) inserted into the left ventricle (LV) via the right common carotid artery. For certain experimental protocols, a tracheostomy was performed, and animals were mechanically ventilated (TOPO ventilator, Kent Scientific, Torrington, CT).
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