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N acetylmuramic acid nam

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N-acetylmuramic acid (NAM) is a naturally occurring chemical compound. It is a component of the cell wall peptidoglycan in many bacteria. NAM serves as a structural element in the peptidoglycan layer.

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Lab products found in correlation

Hemin, by Merck Group (2 mentions) Vitamin k3, by Merck Group (1 mentions) Menadione, by Merck Group (1 mentions) L cysteine, by BD (1 mentions)

3 protocols using n acetylmuramic acid nam

1

Cultivation of Oral Anaerobic Bacteria

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P. gingivalis ATCC 33277 was cultured in brain heart infusion (BHI; BD Biosciences, San Jose, CA, USA) broth supplemented with 5 μg/ml hemin (Sigma-Aldrich, St. Louis, MO, USA) and 1 μg/ml vitamin K3 (menadione; Sigma-Aldrich) under anaerobic conditions (10% CO2, 10% H2, and 80% N2) at 37°C for 48 h. T. denticola ATCC 33521 and T. forsythia ATCC 43037 were cultured in new oral spirochete broth (NOS; ATCC medium 1494) under anaerobic conditions at 37°C for 60 and 48 h, respectively. For T. forsythia, 5 μg/ml hemin, 1 μg/ml vitamin K3, and 10 μg/ml N-acetylmuramic acid (NAM; Sigma-Aldrich) were added.
Lim Y., Kim H.Y., An S.J, & Choi B.K. (2022). Activation of bone marrow-derived dendritic cells and CD4+ T cell differentiation by outer membrane vesicles of periodontal pathogens. Journal of Oral Microbiology, 14(1), 2123550.
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2

Multispecies Biofilm Growth in SHI Medium

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To support the growth of different subpopulations within the pooled saliva, we prepared SHI medium and used it to culture biofilms. The composition of SHI medium was 10 g/l proteose peptone (Difco, Becton Dickinson, Sparks, MD, USA), 5.0 g/l trypticase peptone (Difco), 5.0 g/l yeast extract (Difco), 2.5 g/l mucin (type III, porcine, gastric; Sigma-Aldrich, St. Louis, MO, USA), 10 mg/l N-acetyl muramic acid (NAM, Sigma-Aldrich), 5% sheep blood, 2.5 g/l KCl, 5.0 g/L sucrose, 5.0 mg/l hemin, 1.0 mg/l vitamin K, 0.06 g/l urea and 0.174 g/l arginine41 (link).
Du Q., Fu M., Zhou Y., Cao Y., Guo T., Zhou Z., Li M., Peng X., Zheng X., Li Y., Xu X., He J, & Zhou X. (2020). Sucrose promotes caries progression by disrupting the microecological balance in oral biofilms: an in vitro study. Scientific Reports, 10, 2961.
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3

Anaerobic Growth of Oral Bacteria

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P. gingivalis ATCC 33277 was cultured in 3% tryptic soy broth (TSB; SigmaAldrich, St. Louis, USA), 0.5% yeast extract (YE; Bioshop, Burlington, Canada), 0.0001% menadione (SigmaAldrich), 0.05% L-cysteine (Bioshop) and 0.001% hemin (SigmaAldrich), F. nucleatum ATCC 25586 in 3% TSB, 0.5% YE and 0.05% L-cysteine, T. forsythia ATCC 43037 in 1.85% brain-heart infusion (BHI; BD, Franklin Lakes, USA), 1% YE, 0.0001% menadione, 0.001% hemin and 0.001% N-acetylmuramic acid (NAM; SigmaAldrich), A. naeslundii ATCC 12104 in 1.85% BHI, 1% YE, 0.0001% menadione and 0.001% hemin, and S. gordonii ATCC 10558 in 3% TSB. Agar plates were supplemented with 5% defibrinated sheep’s blood (PWWiU Pro Animali, Wrocław, Poland). Bacteria were cultured under anaerobic conditions (80%, N2, 10% H2 and 10% CO2) at 37°C.
Kamińska M., Aliko A., Hellvard A., Bielecka E., Binder V., Marczyk A., Potempa J., Delaleu N., Kantyka T, & Mydel P. (2018). Effects of Statins on Multispecies Oral Biofilm Identify Simvastatin as A Drug Candidate Targeting Porphyromonas Gingivalis. Journal of periodontology, 90(6), 637-646.
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