Each bacterial strain was cultured to logarithmic phase in THY and collected by centrifugation. Bacterial pellets were fixed in 0.1 M phosphate buffer pH 7.0 containing 2% glutaraldehyde and stained with Alcian blue. Transmission electron microscopy (TEM) was performed as a service by Glenn Hoskins (Department of Pathology, University of Mississippi Medical Center) with a Zeiss TEM LEO 912. Capsule thickness was measured for each strain by selecting a single coccus or diplococcus in a field that appeared representative of all cells for a particular strain, then by taking the mean of a minimum of 10 measurements from the cell wall to the outer edge of the capsule.
Tem leo 912
Manufactured by Zeiss
Sourced in United States
The TEM LEO 912 is a transmission electron microscope (TEM) designed and manufactured by Zeiss. It is a versatile instrument capable of high-resolution imaging and analysis of a wide range of materials at the nanoscale level. The TEM LEO 912 utilizes an electron beam to generate images of thin specimens, allowing for detailed examination of their internal structure and composition.
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Lab products found in correlation
2 protocols using tem leo 912
1
Bacterial Capsule Thickness Quantification
2
Transmission Electron Microscopy of Samples
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Samples were diluted in water, stained with 2% uranyl acetate and imaged by transmission electron microscopy (TEM) at 15,000× and 37,000× magnification. TEM Leo 912 (Zeiss, Thornwood, NY, USA) equipped with a Proscan CCD camera.
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