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M csf

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M-CSF is a recombinant human Macrophage Colony-Stimulating Factor. It is a protein that promotes the differentiation and survival of macrophages, a type of white blood cell involved in the immune response.

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Lab products found in correlation

Mip 2, by Eve Technologies (3 mentions) Bca kit, by Thermo Fisher Scientific (3 mentions) Tnf α, by Eve Technologies (2 mentions) Rantes, by Eve Technologies (2 mentions) G csf, by Eve Technologies (2 mentions) Vascular endothelial growth factor (vegf), by Eve Technologies (2 mentions) Mip 1α, by Eve Technologies (2 mentions) Mip 1β, by Eve Technologies (2 mentions) Ripa buffer, by Thermo Fisher Scientific (2 mentions) K2edta coated tubes, by BD (1 mentions) Gm csf, by Eve Technologies (1 mentions) Mcp 1, by Eve Technologies (1 mentions) Ifn γ, by Eve Technologies (1 mentions) Protease inhibitor, by Thermo Fisher Scientific (1 mentions) Vegf a, by Eve Technologies (1 mentions)

3 protocols using m csf

1

Plasma Cytokine Profiling in Mice

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Blood was collected from the submandibular vein of all experimental mice into K2EDTA-coated tubes (BD Biosciences, 365974) and immediately placed on ice. The blood was centrifuged for 15 minutes at 900g and 4°C, and plasma was collected. Aliquots of plasma were diluted twofold in PBS and stored at –80 °C. Blood plasma cytokines were analysed through a commercially available multiplex panel including Eotaxin, G-CSF, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, CXCL1, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1alpha, MIP-1beta, MIP-2, RANTES, TNF-α, and VEGF (Eve Technologies, Alberta, Canada, MD-31).
McCallion A., Nasirzadeh Y., Lingegowda H., Miller J.E., Khalaj K., Ahn S., Monsanto S.P., Bidarimath M., Sisnett D.J., Craig A.W., Young S.L., Lessey B.A., Koti M, & Tayade C. (2022). Estrogen mediates inflammatory role of mast cells in endometriosis pathophysiology. Frontiers in Immunology, 13, 961599.
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2

Multiplex Cytokine Analysis of Ascites and Plasma

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Ascites fluid and plasma samples collected 24 h following initial STING agonist dose or post carboplatin chemotherapy alone from each treatment group were subjected to multiplex cytokine analysis using the mouse Cytokine Array/Chemokine Array 31-Plex Discovery Assay (includes eotaxin, granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage-colony-stimulating factor (GM-CSF), IFN-γ, interleukin (IL)-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10 (CXCL10), CXCL1, LIF, LIX, MCP-1 (CCL2), macrophage colony-stimulating factor (M-CSF), MIG (CXCL9), MIP-1α, MIP-1β, MIP-2, RANTES (CCL5), tumor necrosis factor-α, and VEGF) at Eve Technologies (Alberta, Canada). All samples were analyzed in biological triplicates. The standard curve regression was used to calculate the concentration of each target cytokine.
Shakfa N., Li D., Conseil G., Lightbody E.D., Wilson-Sanchez J., Hamade A., Chenard S., Jawa N.A., Laight B.J., Afriyie-Asante A., Tyryshkin K., Koebel M, & Koti M. (2023). Cancer cell genotype associated tumor immune microenvironment exhibits differential response to therapeutic STING pathway activation in high-grade serous ovarian cancer. Journal for Immunotherapy of Cancer, 11(4), e006170.
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3

Gastric Cytokine Profiling in Mice

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Gastric tissue from male mice was homogenized in liquid nitrogen with a disposable pestle (Sigma-Aldrich, St. Louis, MO). One hundred fifty microliters of lysis buffer, 500 µL of RIPA buffer with protease inhibitor (Thermo Fisher Scientific, Waltham, MA), 5-µL protease inhibitor, and 5-µL 0.5 M EDTA were added. Samples were placed in a rotating mixer at 4°C for 1 hour. Supernatant was collected following centrifugation at 10,000 × g for 10 minutes at 4°C. Protein concentration was measured using a BCA kit (Thermo Fisher Scientific, Waltham, MA) and adjusted to 1 mg/mL. Thirty-two-plex gastric tissue cytokine array was performed to quantify eotaxin, G-CSF, GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p40, IL-12p70, IL-13, IL-15, IL-17A, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNFα, and VEGF-A (Eve Technologies, Calgary, Alberta, Canada).
Lunger C., Shen Z., Holcombe H., Mannion A.J., Dzink-Fox J., Kurnick S., Feng Y., Muthupalani S., Carrasco S.E., Wilson K.T., Peek R.M., Piazuelo M.B., Morgan D.R., Armijo A.L., Mammoliti M., Wang T.C, & Fox J.G. (2023). Gastric coinfection with thiopeptide-positive Cutibacterium acnes decreases FOXM1 and pro-inflammatory biomarker expression in a murine model of Helicobacter pylori-induced gastric cancer. Microbiology Spectrum, 12(1), e03450-23.
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