1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc

Ammonium bicarbonate (AmBiC), hydroxybenzotriazole hydrate (HOBt), iodoacetamide (IAA), 2-mercaptoethanol, Nonidet P-40 (NP-40), PBS, formic acid (FA), sDHB (2-hydroxy-5-methoxy-benzoic acid and 2,5-dihydroxybenzoic acid, 1:9) as well as a 50% sodium hydroxide (NaOH) solution were obtained from Sigma-Aldrich (Steinheim, Germany). Sodium hydrogen phosphate dihydrate (Na₂HPO₄x2H₂O), sodium bicarbonate, DTT, ethanol, potassium dihydrogen phosphate, SDS, sodium chloride and TFA were purchased from Merck (Darmstadt, Germany). 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) originated from Fluorochem (Hadfield, UK) and HPLC SupraGradient ACN from Biosolve (Valkenswaard, The Netherlands).
Commercial C1-Inh (Cetor®), isolated from pooled cryo- and 4F-depleted plasma, using ion exchange chromatography and PEG precipitation, was obtained from Sanquin, Amsterdam, The Netherlands.

Sodium dodecyl sulphate (SDS), ethanol and trifluoroacetic acid were purchased from Merck (Darmstadt, Germany). Nonidet P-40 substitute (NP-40), super-DHB (9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid)43 , sodium hydroxide (NaOH) and 1-hydroxybenzotriazole hydrate (HOBt) were acquired from Sigma-Aldrich (Steinheim, Germany). 1-Ethyl-3-(3-(dimethylamino)propyl)carbodiimide hydrochloride (EDC) was acquired from Fluorochem (Hadfield United Kingdom). Peptide-N-glycosidase F (PNGase F) was supplied by Roche Diagnostics (Mannheim, Germany). Cotton thread was bought from Pipoos (Utrecht, The Netherlands). HPLC SupraGradient acetonitrile (ACN) was acquired from Biosolve (Valkenswaard, The Netherlands). Peptide calibration standard and an AnchorChip MALDI target plate were purchased from Bruker Daltonics (Bremen, Germany). Pooled plasma from 20 healthy human donors was purchased from Affinity Biologicals (Ancaster, Canada) and used as control sample. Lastly, water was purified with a Purelab Ultra from Elga LabWater (Ede, The Netherlands).