Protein extracts (25 to 30 μg) from different cells, as well as treated mBMCs, were subjected to SDS-PAGE and immunoblotting using standard methods. Anti-CXCR4 and anti-α-tubulin antibodies used were from Santa Cruz Biotechnology (Dallas, USA) while secondary antibody conjugated with horseradish peroxidase was obtained from Cell Signaling (Beverly, Massachusetts). The CXCR4 protein band densities were quantified using Quantity One software from BioRad and relative band density was calculated as a ratio of sample to α-tubulin.
Anti cxcr4 and anti α tubulin antibodies
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-CXCR4 and anti-α-tubulin antibodies are laboratory reagents used for research purposes. The anti-CXCR4 antibody is a tool for identifying and studying the CXCR4 protein, which is a chemokine receptor. The anti-α-tubulin antibody is a tool for identifying and studying the α-tubulin protein, which is a component of the cytoskeleton. These antibodies can be used in various immunological techniques, such as Western blotting, immunohistochemistry, and flow cytometry.
Automatically generated - may contain errors
2 protocols using anti cxcr4 and anti α tubulin antibodies
1
Quantifying CXCR4 Protein Expression
2
Quantifying CXCR4 Protein Expression
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