Blood samples were taken from the forearm vein before exercise, and 24 h, 48 h, 72 h, and 96 h after exercise to obtain blood CK and Mb levels. A vacutainer system was used for the sampling, and 5 mL of blood was taken for each sampling. The sample contained in a vacutainer SST was centrifuged at 2500–3000 rpm for 10–15 min. Then, the sample was tested for CK by placing the reagent (Vitros 750, Johnson and Johnson, Phoenix, AZ, USA) in an automated blood analyzer (Ortho Johnson Vitros DT60 II, Johnson and Johnson, Phoenix, AZ, USA). The sample was tested for Mb by placing the reagent (LIAISON Myoglobin, DiaSorin, Saluggia, Italy) in clinical test equipment (LIAISON, DiaSorin, Saluggia, Italy).
Vitros dt60 2
Manufactured by Johnson & Johnson
Sourced in United States
The Vitros DT60 II is a diagnostic instrument designed for automated clinical chemistry analysis. It is capable of performing a wide range of tests on various sample types, providing accurate and reliable results for healthcare professionals.
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Lab products found in correlation
7 protocols using vitros dt60 2
1
Exercise-Induced Muscle Damage Biomarkers
2
Pancreatic Tissue and Blood Analysis
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The study was terminated at the end of 12 weeks post treatment by slaughtering through electrical stunning and exsanguination and the entire pancreas was obtained and weighed after towel cleaning. Each pancreas was incised to obtain 3 samples (1 cm3 in size) from the anterior (close to duodenum), median (close to superior mesenteric artery) and the most posterior sites. The obtained tissues were fixed in 10% formalin immediately. All of the blood samples were centrifuged and the obtained plasma was stored in vials at −80℃ until analysis.
The blood clinical profile was analysed using a VITROS DT60II and a DTSC II (Johnson & Johnson Clinical Diagnostics, Inc., USA), and glucose, triglycerides, cholesterol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, aspartate aminotransferase, alanine aminotransferase, urea nitrogen and creatinine measurements were performed. The plasma insulin and fructosamine concentrations were determined using enzyme-linked immunosorbent assay kits designed for pig insulin (Shibayagi, Japan) and fructosamine (EIAab® Science, Co., Ltd., China). The homeostatic model assessment-insulin resistance index (HOMA-IR) was calculated using the equation HOMA-IR=fasting blood insulin (µIU/mL)×fasting blood glucose (mmol/L)/22.5 [16 (link)].
The blood clinical profile was analysed using a VITROS DT60II and a DTSC II (Johnson & Johnson Clinical Diagnostics, Inc., USA), and glucose, triglycerides, cholesterol, low density lipoprotein-cholesterol, high density lipoprotein-cholesterol, aspartate aminotransferase, alanine aminotransferase, urea nitrogen and creatinine measurements were performed. The plasma insulin and fructosamine concentrations were determined using enzyme-linked immunosorbent assay kits designed for pig insulin (Shibayagi, Japan) and fructosamine (EIAab® Science, Co., Ltd., China). The homeostatic model assessment-insulin resistance index (HOMA-IR) was calculated using the equation HOMA-IR=fasting blood insulin (µIU/mL)×fasting blood glucose (mmol/L)/22.5 [16 (link)].
3
Centrifugation, Serum Analyte Analysis
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Blood samples from the jugular vein were centrifuged 4 hours after collection at 3,000× g for 15 minutes (Sorvall® ST 16; Thermo Fisher Scientific, Waltham, MA, USA) to obtain blood serum. The activity of alanine aminotransaminase, asparagine aminotransaminase, lactate dehydrogenase, and alkaline phosphatase and the levels of glucose and blood urea nitrogen were examined using the dry chemistry equipment Vitros® DT 60 II (Johnson & Johnson, New Brunswick, NJ, USA) with Manuals Maintenance and Troubleshooting (Johnson & Johnson).
4
Anesthesia and Serum Biochemistry
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Animals were anesthetized using intramuscular 20 mg/kg Zoletil. Blood samples were collected through intracardiac puncture before sacrifice and centrifuged at 1500× g, 4 °C, for 10 min to obtain serum. Serum aliquots were stored at −80 °C. Serum levels of AST and ALT were quantified using a VITROS DT60II dry chemistry analyzer (Johnson & Johnson, Nuevo Brunswick, NJ, USA).
5
Hepatic Enzyme Quantification in Blood
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Blood samples were centrifuged (3000 rpm, 15 min; Thermo Fisher Scientific, Waltham, MA, USA) and activities of alanine aminotransferase (ALT) and asparagine aminotransferase (AST) were measured. ALT and AST were examined using the Vitros DT 60 II chemistry system (Johnson and Johnson, New Brunswick, NJ, USA).
6
Serum CK Activity Measurement Protocol
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Blood samples were taken from the antecubital vein using a collection tube (BD Vacutainer, BD Plymouth, Plymouth, UK) and were stored at room temperature for 20 min, in orderto obtainserum. They were then centrifuged at 3,000 rpm for 10 min, and the serum was pipetted and separated into a 1.5-mL microtube (MCT-150-C, Axygen, a subsidary of Corning, New York, NY, USA). Each sample was stored at −80°C for analysis of CK activity using an automated analyzer (Vitros DT60 II, Johnson & Johnson, Minneapolis, MN, USA).
7
Blood Pressure and Lipid Profile Assessment
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